Fine Mapping of qHD4-1, a QTL Controlling the Heading Date, to a 20.7-kb DNA Fragment in Rice (Oryza sativa L.)

文献类型： 外文期刊

第一作者： Wang, Binbin

作者： Wang, Binbin;Liu, Xu;Wang, Wenying;Ding, Hanfeng;Liu, Wei;Yao, Fangyin;Jiang, Mingsong;Li, Guangxian;Zhu, Changxiang

作者机构：

关键词： Oryza sativa; Heading date; Single-segment substitution line (SSSL); Physical mapping; Sequence information

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN： 0735-9640

年卷期： 2011 年 29 卷 3 期

页码：

收录情况： SCI

摘要： A library consisting of 1,123 single-segment substitution lines (SSSLs) in the same genetic background of an elite rice variety Huajingxian74 (HJX74) was evaluated for heading date. From this library, the SSSL W05-1-11-5-16-2-5 with the substituted interval of PSM103-RM348-OSR15-PSM382-RM131-RM127-RM280 was found having a gene, which stably performed extreme late heading date which performed stable and late heading in the different environments of Shandong, Guangdong, and Hainan. To map the gene governing heading date, the SSSL W05-1-11-5-16-2-5 was crossed with the recipient HJX74 to develop an F(2) segregating population. The distribution of late and early heading plants in this population fitted a segregation ratio of 3:1, indicating the late heading was controlled by a dominant gene. The gene locus for heading date was tentatively designated as qHD4-1. Using a random sample of 460 individuals from the F(2) segregation population, the qHD4-1 locus was mapped between two SSR markers RM3335 and RM17572, with genetic distances of 0.1 and 0.2 cM, respectively. For fine mapping of qHD4-1, a large F(2:3) segregating population of 3,000 individuals were developed from F(2) plants heterozygous in the RM3335-RM17572 region. Recombinants analyses further mapped qHD4-1 to an interval of 20.7-kb-bounded WB05 and the WB06. Sequence analysis of this 20.7-kb region revealed that it contains three open reading frames (ORFs), encoding walla-ssociated receptor kinase-like 5, putative F-box domain containing protein, and putative arogenate/prephenate dehydratase. Of them, ORF1, predicting to encode serine/threonine kinase, is considered the most likely as the candidate gene. The genetic and physical map of the qHD4-1 locus will be very useful in molecular cloning of the qHD4-1 gene.

分类号： Q94

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