Intermolecular Structure Conversion-Based G4-TDF Nanostructures Functionalized μPADs for Fluorescent Determination of Potassium Ion in Serum

文献类型： 外文期刊

第一作者： Wang, Mengqi

作者： Wang, Mengqi;Fu, Xiuli;Liu, Yixuan;Zhang, Zhiyang;Jiang, Chenyu;Song, Dean

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关键词： G-quadruplex; DNA tetrahedral nanostructure; structure conversion; thioflavin T; potassium ion

期刊名称：BIOSENSORS-BASEL （ 影响因子：5.6； 五年影响因子：5.7 ）

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年卷期： 2025 年 15 卷 4 期

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收录情况： SCI

摘要： Herein, we proposed a versatile G-quadruplex (G4)-tetrahedral DNA framework (G4-TDF) nanostructure functionalized origami microfluidic paper-based device (mu PADs) for fluorescence detection of K+ by lighting up thioflavin T (ThT). In this work, TDF provided robust structural support for G-rich sequence in well-defined orientation and spacing to ensure high recognition efficiency, enabling sensitive fluorescence sensing on origami mu PAD. After introducing ThT, the G-rich sequences extended from TDF vertices formed a parallel G4 structure, showing weak fluorescence signal output. Upon the presence of target K+, this parallel G4 structure transitioned to antiparallel G4 structure, leading to a significantly increase in fluorescence signal of ThT. Benefiting from the outstanding fluorescence enhancement characteristic of the G4 structure for ThT and excellent specificity of the G4 structure to K+ plus satisfactory recognition efficiency with the aid of TDF, this origami paper-based fluorescence sensing strategy exhibited an impressive detection limit as low as 0.2 mM with a wide range of 0.5-5.5 mM. This innovative G4-TDF fluorescence sensing was applied for the first time on mu PAD, providing a simple, effective, and rapid method for K+ detection in human serum with significant potential for clinical diagnostics.

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