The MGF300-2R Protein of African Swine Fever Virus Promotes IKKβ Ubiquitination by Recruiting the E3 Ubiquitin Ligase TRIM21

文献类型： 外文期刊

第一作者： Lu, Zhanhao

作者： Lu, Zhanhao;Luo, Rui;Lan, Jing;Qiu, Hua-Ji;Wang, Tao;Sun, Yuan;Lan, Jing;Qiu, Hua-Ji;Chen, Shengmei;Qiu, Hua-Ji

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关键词： African swine fever virus; MGF300-2R; E3 ubiquitin ligase; TRIM21; ubiquitin; IKK beta

期刊名称：VIRUSES-BASEL （ 影响因子：3.8； 五年影响因子：4.0 ）

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年卷期： 2024 年 16 卷 6 期

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收录情况： SCI

摘要： African swine fever (ASF) is an acute, hemorrhagic, highly contagious disease in pigs caused by African swine fever virus (ASFV). Our previous study identified that the ASFV MGF300-2R protein functions as a virulence factor and found that MGF300-2R degrades IKK beta via selective autophagy. However, the E3 ubiquitin ligase responsible for IKK beta ubiquitination during autophagic degradation still remains unknown. In order to solve this problem, we first pulled down 328 proteins interacting with MGF300-2R through immunoprecipitation-mass spectrometry. Next, we analyzed and confirmed the interaction between the E3 ubiquitin ligase TRIM21 and MGF300-2R and demonstrated the catalytic role of TRIM21 in IKK beta ubiquitination. Finally, we indicated that the degradation of IKK beta by MGF300-2R was dependent on TRIM21. In summary, our results indicate TRIM21 is the E3 ubiquitin ligase involved in the degradation of IKK beta by MGF300-2R, thereby augmenting our understanding of the functions of MGF300-2R and offering insights into the rational design of live attenuated vaccines and antiviral strategies against ASF.

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