The comparative study revealed that the hTERT-CSF cell line was the most susceptible cell to the Lumpy skin disease virus infection among eleven cells

文献类型： 外文期刊

第一作者： Ma, Chunling

作者： Ma, Chunling;Ren, Shanhui;Afera, Tadele Berihun;Yang, Xue;Lin, Yugang;Gao, Xiaohong;Wang, Fangping;Wang, Xiangwei;Yin, Xiangping;Sun, Yuefeng;Chen, Hao-tai;Ma, Chunling;Wang, Fangping;Wan, Xuerui;Lin, Yugang;Qiu, Xusheng

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关键词： Comparative study; Susceptibility; Lumpy skin disease virus; HTERT-CSF

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：3.1； 五年影响因子：2.4 ）

ISSN： 0166-0934

年卷期： 2023 年 317 卷

页码：

收录情况： SCI

摘要： Lumpy skin disease virus (LSDV) is a rapidly emerging pathogen in Asia, including China. Improving the propagation of LSDV is important for diagnostics and vaccine production. Our study identified and compared the LSDV susceptibility of eleven standard cells using western blot, indirect immune-fluorescence assay, quantitative PCR, and 50 % tissue culture infectious dose. Our finding revealed that the LSDV strain could infect five cell lines and show a cytopathic effect. Furthermore, the hTERT-CSF cell line had the highest level of virus in the five cell models, followed by BHK-21, MDBK, Vero, and hTERT-ST. Hence, hTERT-CSF could be used as a candidate cell line for basic and applied research, clinical application, and LSDV vaccine development, providing a vital reference in LSDV and other viruses.

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