Identification of an adaptor protein 2σ gene for OsTGW12 to determine grain weight and potentiate quality breeding in rice

文献类型： 外文期刊

第一作者： Li, Xiaoqiong

作者： Li, Xiaoqiong;Liu, Kaiqiang;Chen, Ying;Qi, Jingang;Guo, Sibin;Li, Huangai;Xie, Ke;Jin, Hongpei;Han, Jinling;Sha, Aihua;Tong, Hanhua

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：4.2； 五年影响因子：4.9 ）

ISSN： 0040-5752

年卷期： 2025 年 138 卷 9 期

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收录情况： SCI

摘要： Key messageThis study identified an adaptor protein gene OsAP2 sigma (LOC_Os12g10560) as a causal gene for OsTGW12, a major QTL on rice chromosome to determine the thousand-grain weight (TGW), elucidated the role of a specific SNP (G266A) within this gene in determining grain size by affecting cell number, and the demonstrated interaction between OsAP2 sigma and OsSAMS1 suggested a novel regulatory mechanism linking clathrin-mediated endocytosis (CME) to S-adenosylmethionine metabolism in rice grain development.AbstractIn rice, many quantitative trait loci/genes of thousand-grain weight (TGW) have been identified and applied to breed rice varieties with high yield. Here, we identified an allele OsAP2 sigma 89G from a small grain indica rice variety G1025, a causal gene for OsTGW12 on rice chromosome 12 to determine grain weight. AP2 sigma is an adaptor protein (AP) subunit 2 sigma involved in the clathrin-mediated endocytosis (CME) in eukaryotic cells. Moreover, overexpression of the allele K1561-OsAP2 sigma 89E but not the allele G1025-OsAP2 sigma 89G increased grain length, grain width and grain weight than the wild type G1025 whereas the RNAi-mediated OsAP2 sigma mutant showed the opposites. Co-immunoprecipitation mass spectrometry (Co-IP-MS), yeast two-hybrid (Y2H), and luciferase implementation (LUC) assays demonstrated the interaction between OsAP2 sigma and S-adenosylmethionine synthetase (OsSAMS1), a key enzyme involved in methionine metabolism pathway. In addition, both OsAP2 sigma and OsSAMS1 were localized into the plasma membrane (PM) and nucleus. Transcriptomic analysis revealed that OsAP2 sigma regulated the expressions of related genes to CME, mitogen activated protein kinase (MAPK) signaling, and auxin transport and transcriptional regulation during rice grain development. Thus, this study reveals a new role of OsAP2 sigma and provides a potential in further breeding programs of the quality improvement in rice.

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