Transfer of bacterial blight resistance from Oryza meyeriana to O. sativa L. by asymmetric somatic hybridization

文献类型： 外文期刊

第一作者： Zhu, YS

作者： Zhu, YS;Chen, BT;Yu, SW;Zhang, DP;Zhang, XQ;Yan, QS

作者机构：

关键词： somatic hybridization;Oryza saliva L.;Oryza meyeriana;rice bacterial blight.

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： Asymmetric somatic hybrid plants were produced between cultivated rice (Oryza saliva L.) and wild species [0. meyeriana (Zoll. etMor. exSteud.)] with high resistance to rice bacterial blight. X-ray-irradiated protoplasts of the wild species were used as donor and chemically fused with iodoacetamide-inactivated protoplasts of rice cv. 02428 to produce hybrids. Seventy-two plants were regenerated from 623 calli based on metabolic complementation. The morphological characters of the plants closely resembled that of the rice. Simple sequence repeats were employed to identify their hybridity. Cytological analysis of root-tips revealed that their chromosome number varied in the range of 27-- 38. The somatic hybrids were inoculated with strains of Xanthamonas oryzae pv. oryzae at adult growth stage and demonstrated the resistance to bacterial blight introgression from the O.meyeriana,

分类号： N1

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