Magnetic Janus SiO2 nanoparticles immobilized protease mutant T70I as a novel clarification agent for juice processing

文献类型： 外文期刊

第一作者： Herman, Richard Ansah

作者： Herman, Richard Ansah;Zhu, Xuan;Khurshid, Marriam;Zhang, Zhan-Peng;You, Shuai;Wang, Jun;Herman, Richard Ansah;You, Shuai;Wang, Jun;Ayepa, Ellen;Qian, Jun-Feng;Qian, Jun-Feng;Wang, Jun

作者机构：

关键词： Mucor circinelloides aspartic protease; Magnetic Janus silica nanoparticle; Juice clarification

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 292 卷

页码：

收录情况： SCI

摘要： The juice processing industry offers several benefits, including promoting health and wellness through the delivery of beverages rich in nutrients. Nonetheless, the industry encounters significant challenges regarding new technologies for processing and preservation given that they can be costly and labor-intensive. In this study, magnetic Janus silica (SiO2) nanoparticle which offers multifunctionality and high stability was synthesized and subsequently immobilized with a protease mutant T70I (T70I@MSNs) to serve as a clarifying agent in juice. The optimal conditions for the protease immobilized with SiO2 nanoparticles showed superior tolerance and stability at pH 5 and 65 degrees C. Box-Behnken Design revealed optimizing conditions of 60 min, 50 degrees C and 0.2 % enzyme concentration which significantly influenced the clarification of the juice samples. Immobilized T70I@MSNs decreased >70 % of juice turbidity while maintaining about 78 % of enzyme activity after 7 consecutive cycles of reuse. Notably, T70I@MSN treatment enhanced certain volatile components by increasing alcohol and ester production, while changes in surface microstructure and functional groups were observed. This study highlights the significant improvement of magnetic Janus SiO2 nanoparticles immobilized proteases in the clarification of juice against haze and undesirable properties, thereby offering a notable contribution to the juice processing industry.

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