A cluster of mutagenesis revealed an osmotic regulatory role of the OsPIP1 genes in enhancing rice salt tolerance

文献类型： 外文期刊

第一作者： Tao, Leyuan

作者： Tao, Leyuan;Wang, Bing;Li, Wei;Huang, Shengcai;Zhang, Qianru;Cheng, Xianguo;Tao, Leyuan;Wang, Bing;Liu, Laihua;Xin, Shichao;Cui, Jing

作者机构：

关键词： Aquaporin; Multiplex gene editing; CRISPR/Cas9; OsPIP1 genes; Salt tolerance; Rice(Oryza sativa L.)

期刊名称：CROP JOURNAL （ 影响因子：6.6； 五年影响因子：6.5 ）

ISSN： 2095-5421

年卷期： 2023 年 11 卷 4 期

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收录情况： SCI

摘要： Aquaporins play important regulatory roles in improving plant abiotic stress tolerance. To better understand whether the OsPIP1 genes collectively dominate the osmotic regulation in rice under salt stress, a cluster editing of the OsPIP1;1, OsPIP1;2 and OsPIP1;3 genes in rice was performed by CRISPR/Cas9 system. Sequencing showed that two mutants with Cas9-free, line 14 and line 18 were successfully edited. Briefly, line 14 deleted a single C base in both the OsPIP1;1 and OsPIP1;3 genes, and inserted a single T base in the OsPIP1;2 gene, respectively. While line 18 demonstrated an insertion of a single A base in the OsPIP1;1 gene and a single T base in both the OsPIP1;2 and OsPIP1;3 genes, respectively. Multiplex editing of the OsPIP1 genes significantly inhibited photosynthetic rate and accumulation of compatible metabolites, but increased MDA contents and osmotic potentials in the mutants, thus delaying rice growth under salt stress. Functional loss of the OsPIP1 genes obviously suppressed the expressions of the OsPIP1, OsSOS1, OsCIPK24 and OsCBL4 genes, and increased the influxes of Na' and effluxes of K'/H' in the roots, thus accumulating more Na' in rice mutants under salt stress. This study suggests that the OsPIP1 genes are essential modulators collectively contributing to the enhancement of rice salt stress tolerance, and multiplex editing of the OsPIP1 genes provides insight into the osmotic regulation of the PIP genes. (c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).

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