A Sensitive and Specific Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Pretilachlor in Grains and the Environment

文献类型： 外文期刊

第一作者： Zhang, Linwei

作者： Zhang, Linwei;Yin, Xiaoyang;Yang, Hongfei;Wen, Hao;Han, Shiyun;Pan, Xiaoming;Li, Huaming;Peng, Dapeng;Zhang, Linwei;Yin, Xiaoyang;Yang, Hongfei;Wen, Hao;Han, Shiyun;Pan, Xiaoming;Li, Huaming;Peng, Dapeng;Peng, Dapeng;Peng, Dapeng;Peng, Dapeng

作者机构：

关键词： residue detection; pretilachlor; monoclonal antibody; ic-ELISA

期刊名称：FOODS （ 影响因子：5.2； 五年影响因子：5.5 ）

ISSN：

年卷期： 2024 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Pretilachlor is a chloroacetamide herbicide mainly used for weed and broadleaf weed control in rice, that is widely utilized in China. In order to detect the residue of pretilachlor in the environment and food, a highly sensitive and specific monoclonal antibody (mAb) against pretilachlor was prepared, and the half maximum inhibitory concentration (IC50) of the monoclonal antibody was validated to be 31.47 +/- 2.35 mu g/L. An indirect competitive ELISA (ic-ELISA) based on the antibody with a linear range of 6.25 similar to 100 mu g/L was developed. The specificity of the antibody was explained by computer simulations and experimental validation. The mAb exhibited negligible cross-reactivity towards alachlor, acetochlor, propisochlor, butachlor, and metalaxyl, and the limits of detection (LOD) for pretilachlor in lake, rice, and soil samples were 4.83 similar to 5.23 mu g/L. The recoveries of all samples were 78.3 similar to 91.3%. The reliability of the ic-ELISA method for residue detection of pretilachlor in the environment and grains was confirmed using high performance liquid chromatography.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序