Sus scrofa RNase L inhibits PRRSV replication by activation of type I IFN signaling pathway and apoptosis

文献类型： 外文期刊

第一作者： Wu, Xiangju

作者： Wu, Xiangju;Cong, Xiaoyan;Zhou, Mingming;Yu, Ying;Jiang, Dandan;Hu, Yue;Li, Juntong;Zhang, Jinxia;Cao, Ying;Zhang, Haowen;Du, Yijun;Qi, Jing;Jiang, Ping

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关键词： Sus scrofa RNase L; Porcine reproductive and respiratory syndrome; virus; Antiviral; Innate immunity; 2-5 A

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）

ISSN： 0378-1135

年卷期： 2025 年 302 卷

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收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) has become one of the most economically important diseases to the global pig industry. RNase L is a ubiquitous cellular endoribonuclease that is activated upon the binding of a specific ligand, 2 ',5 '-linked oligoadenylates (2-5 A), which is synthesized by oligoadenylate synthetases (OASs). However, whether Sus scrofa RNase L (sRNase L) could inhibit PRRSV replication and its mechanism have not been fully elucidated. In this study, sRNase L was cloned and characterized in homology and structure firstly. Then the antiviral activity of sRNase L against PRRSV was explored. Overexpression of sRNase L significantly inhibited the propagation of PRRSV when treated with 2-5 A or poly(I: C) or mock treated. Furthermore, sRNase L induced degradation of cellular and viral ssRNAs, enhanced the activation of IFN-beta promoter and IFN-beta expression, and induced apoptosis to inhibit PRRSV replication. Taken together, we have first elucidated the anti-PRRSV function and the underlying mechanism of sRNase L, which may provide a new strategy for preventing PRRSV infection.

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