Enantioselective activity and toxicity of chiral acaricide cyflumetofen toward target and non-target organisms

文献类型： 外文期刊

第一作者： Zhang, Yifan

作者： Zhang, Yifan;Li, Lin;Yang, Lin;Zhao, Mengying;Jin, Nuo;Wang, Fengzhong;Fan, Bei;Li, Minmin;Kong, Zhiqiang;Gregoire, Noel;Francis, Frederic

作者机构：

关键词： Cyflumetofen; Enantioselective toxicity; MCF-7; Honeybees; Estrogen

期刊名称：CHEMOSPHERE （ 影响因子：8.8； 五年影响因子：8.3 ）

ISSN： 0045-6535

年卷期： 2023 年 325 卷

页码：

收录情况： SCI

摘要： Cyflumetofen (CYF), a novel chiral acaricide, exert enantiomer-specific effects on target organisms by binding to glutathione S-transferase. However, there is limited knowledge regarding the response of non-target organisms to CYF, including enantioselective toxicity. In this study, we investigated the effects of racemic CYF (rac-CYF) and its two enantiomers (+)-CYF and (-)-CYF on MCF-7 cells and non-target (honeybees) and target (bee mites and red spider mites) organisms. The results showed that similar to estradiol, 1 mu M (+)-CYF promoted the prolif-eration and disturbed the redox homeostasis of MCF-7 cells, whereas at high concentrations (>= 100 mu M) it exerted a negative effect on cell viability that was substantially stronger than that of (-)-CYF or rac-CYF. (-)-CYF and rac-CYF at 1 mu M concentration did not significantly affect cell proliferation, but caused cell damage at high concentrations (>= 100 mu M). Analysis of acute CYF toxicity against non-target and target organisms revealed that for honeybees, all CYF samples had high lethal dose (LD50) values, indicating low toxicity. In contrast, for bee mites and red spider mites, LD50 values were low, whereas those of (+)-CYF were the lowest, suggesting higher toxicity of (+)-CYF than that of the other CYF samples. Proteomics profiling revealed potential CYF-targeted proteins in honeybees related to energy metabolism, stress responses, and protein synthesis. Upregulation of estrogen-induced FAM102A protein analog indicated that CYF might exert estrogenic effects by dysregulating estradiol production and altering estrogen-dependent protein expression in bees. Our findings suggest that CYF functions as an endocrine disruptor in non-target organisms in an enantiomer-specific manner, indicating the necessity for general ecological risk assessment for chiral pesticides.

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