Detection and identification of SARS-CoV-2 and influenza a based on microfluidic technology

文献类型： 外文期刊

第一作者： Liu, Yujie

作者： Liu, Yujie;Yu, Guanliu;Liang, Hongkun;Li, Hua;Chen, Lei;Sun, Wenbo;Zhang, Lulu;Mauk, Michael G.

作者机构：

期刊名称：ANALYTICAL METHODS （ 影响因子：2.7； 五年影响因子：2.7 ）

ISSN： 1759-9660

年卷期： 2024 年 16 卷 27 期

页码：

收录情况： SCI

摘要： As of now, the global COVID-19 pandemic caused by SARS-CoV-2, which began in 2019, has been effectively controlled. However, the symptoms of influenza A virus infection were similar to those of SARS-CoV-2 infection, but they required different treatment approaches. To make the detection more accurate and the treatment more targeted. We developed a system that integrates RPA and CRISPR assays, allowing for the rapid, highly specific, and sensitive detection and differentiation of SARS-CoV-2, H1N1, and H3N2. Under isothermal amplification conditions, the RPA-CRISPR Cas12a detection system achieved a detection limit as low as 5 copies per mu L, demonstrating excellent specificity. The measurement time was approximately 30 minutes. The RPA-CRISPR Cas12a detection system combined with the microfluidic chip we designed to simultaneously detect three viruses, providing a potential solution for efficient and reliable diagnosis. We developed a system that integrates RPA-CRISPR Cas12a with microfluidic chip, allowing for the rapid, highly specific, and sensitive detection and differentiation of SARS-CoV-2, H1N1, and H3N2.

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