Transcriptome-Wide Analysis of RNA m(6)A Methylation and Gene Expression Changes Among Two Arabidopsis Ecotypes and Their Reciprocal Hybrids

文献类型： 外文期刊

第一作者： Xu, Zhihui

作者： Xu, Zhihui;Xu, Zhihui;Shi, Xiaobo;Bao, Mengmei;Song, Xiaoqian;Zhang, Yuxia;Wang, Haiyan;Xie, Hairong;Mao, Fei;Wang, Shuai;Wu, Yufeng;Jin, Hongmei;Dong, Suomeng;Zhang, Feng;Wu, Zhe

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关键词： RNA m(6)A methylation; hybrid; heterosis; Arabidopsis; RNA modification dynamics

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.754； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： The remodeling of transcriptome, epigenome, proteome, and metabolome in hybrids plays an important role in heterosis. N(6)-methyladenosine (m(6)A) methylation is the most abundant type of post-transcriptional modification for mRNAs, but the pattern of inheritance from parents to hybrids and potential impact on heterosis are largely unknown. We constructed transcriptome-wide mRNA m(6)A methylation maps of Arabidopsis thaliana Col-0 and Landsberg erecta (Ler) and their reciprocal F-1 hybrids. Generally, the transcriptome-wide pattern of m(6)A methylation tends to be conserved between accessions. Approximately 74% of m(6)A methylation peaks are consistent between the parents and hybrids, indicating that a majority of the m(6)A methylation is maintained after hybridization. We found a significant association between differential expression and differential m(6)A modification, and between non-additive expression and non-additive methylation on the same gene. The overall RNA m(6)A level between Col-0 and Ler is clearly different but tended to disappear at the allelic sites in the hybrids. Interestingly, many enriched biological functions of genes with differential m(6)A modification between parents and hybrids are also conserved, including many heterosis-related genes involved in biosynthetic processes of starch. Collectively, our study revealed the overall pattern of inheritance of mRNA m(6)A modifications from parents to hybrids and a potential new layer of regulatory mechanisms related to heterosis formation.

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