Isolation and Characterization of SPOTTED LEAF42 Encoding a Porphobilinogen Deaminase in Rice

文献类型： 外文期刊

第一作者： Liu, Lin

作者： Liu, Lin;Wang, Yunpeng;Tian, Yunlu;Song, Shuang;Wu, Zewan;Ding, Xin;Zheng, Hai;Huang, Yunshuai;Liu, Shijia;Dong, Xiaoou;Wan, Jianmin;Liu, Linglong;Wan, Jianmin

作者机构：

关键词： chlorophyll biosynthesis; chloroplast; rice (Oryza sativa L; ); RNA editing; spotted leaf; porphobilinogen deaminase (PBGD)

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 12 卷 2 期

页码：

收录情况： SCI

摘要： The formation and development of chloroplasts play a vital role in the breeding of high-yield rice (Oryza sativa L.). Porphobilinogen deaminases (PBGDs) act in the early stage of chlorophyll and heme biosynthesis. However, the role of PBGDs in chloroplast development and chlorophyll production remains elusive in rice. Here, we identified the spotted leaf 42 (spl42) mutant, which exhibited a reddish-brown spotted leaf phenotype. The mutant showed a significantly lower chlorophyll content, abnormal thylakoid morphology, and elevated activities of reactive oxygen species (ROS)-scavenging enzymes. Consistently, multiple genes related to chloroplast development and chlorophyll biosynthesis were significantly down-regulated, whereas many genes involved in leaf senescence, ROS production, and defense responses were upregulated in the spl42 mutant. Map-based cloning revealed that SPL42 encodes a PBGD. A C-to-T base substitution occurred in spl42, resulting in an amino acid change and significantly reduced PBGD enzyme activity. SPL42 targets to the chloroplast and interacts with the multiple organelle RNA editing factors (MORFs) OsMORF8-1 and OsMORF8-2 to affect RNA editing. The identification and characterization of spl42 helps in elucidating the molecular mechanisms associated with chlorophyll synthesis and RNA editing in rice.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序