A role of peptidoglycan recognition protein in mediating insecticide detoxification in Glyphodes pyloalis

文献类型： 外文期刊

第一作者： Jiang, De-Lei

作者： Jiang, De-Lei;Ding, Jian-Hao;Liu, Zhi-Xiang;Shao, Zuo-Ming;Liang, Xin-Hao;Wang, Jun;Wu, Fu-An;Sheng, Sheng;Wang, Jun;Wu, Fu-An;Sheng, Sheng

作者机构：

关键词： chlorfenapyr; detoxification genes; Glyphodes pyloalis; peptidoglycan recognition protein; phoxim

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

ISSN： 0739-4462

年卷期： 2021 年 108 卷 3 期

页码：

收录情况： SCI

摘要： Glyphodes pyloalis Walker has become one of the most significant mulberry pests, and it has caused serious economic losses in major mulberry growing regions in China. Peptidoglycan recognition proteins (PGRPs) are responsible for initiating and regulating immune signalling pathways in insects. However, their roles responding to chemical pesticides is still less known. This study aimed to investigate the possible detoxication function of GpPGRP-S2 and GpPGRP-S3 in G. pyloalis in response to chlorfenapyr and phoxim. The chlorfenapyr and phoxim treatment significantly induced the expression level of GpPGRP-S3 at 48 h. In addition, the expression levels of GpPGRP-S2 and GpPGRP-S3 in the chlorfenapyr/phoxim treatment group were significantly higher in midgut than those in the control group at 48 h. The results of the survival experiment showed that silencing either GpPGRP-S2 or GpPGRP-S3 would not influence the survival rate of G. pyloalis which treated with phoxim, however, silencing GpPGRP-S2 or GpPGRP-S3 would cause G. pyloalis to be more easily killed by chlorfenapyr. The expression of carboxylesterase GpCXE1 was significantly induced by chlorfenapyr/phoxim treatment, while it was suppressed once silenced GpPGRP-S2 followed with chlorfenapyr treatment or silenced GpPGRP-S3 followed with phoxim treatment. These results might suggest that under the chlorfenapyr/phoxim treatment condition, the connection between GpPGRPs and detoxification genes in insect was induced to maintain physiological homeostasis; and these results may further enrich the mechanisms of insects challenged by insecticides.

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