A universal CRISPR/Cas12a-powered intelligent point-of-care testing platform for multiple small molecules in the healthcare, environment, and food

文献类型： 外文期刊

第一作者： Zhao, Yuan

作者： Zhao, Yuan;Wu, Wenqin;Tang, Xiaoqian;Zhang, Qi;Mao, Jin;Yu, Li;Li, Peiwu;Zhang, Zhaowei;Zhang, Qi;Mao, Jin;Li, Peiwu;Zhang, Zhaowei;Li, Peiwu;Zhang, Zhaowei

作者机构：

关键词： CRISPR; Cas12a; Intelligent point -of -care testing; Multiple small molecules detection; Healthcare test; Environment monitoring; Food safety

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：12.6； 五年影响因子：10.8 ）

ISSN： 0956-5663

年卷期： 2023 年 225 卷

页码：

收录情况： SCI

摘要： Growing studies focusing on nuclear acid detection via the emerging CRISPR technique demonstrate its prom-ising application. However, limited works solve the identification of non-nucleic acid targets, especially multiple small molecules. To address challenges for point-of-care testing (POCT) in complex matrices for healthcare, environment, and food safety, we developed CRISPR Cas12a-powered highly sensitive, high throughput, intel-ligent POCT (iPOCT) for multiple small molecules based on a smartphone-controlled reader. As a proof of concept, aflatoxin B1 (AFB1), benzo[a]pyrene (BaP), and capsaicin (CAP) were chosen as multiple targets. First, three antigens were preloaded in independent microwells. Then, the antibody/antigen-induced fluorescent sig-nals were consecutively transferred from the biotin-streptavidin to CRISPR/Cas12a system. Third, the fluorescent signals were recorded by a smartphone-controlled handheld dark-box readout. Under optimization, detection limits in AFB1, BaP, and CAP were 0.00257, 4.971, and 794.6 fg/mL with wide linear ranges up to four orders of magnitude. Using urine, water, soybean oil, wheat, and peanuts as the complex matrix, we recorded high selectivity, considerable recovery, repeatability, and high consistency comparison to HPLC-MS/MS methods. This work promises a practical intelligent POCT platform for multiple targets in lipid-soluble and water-soluble matrices and could be extensively applied for healthcare, environment, and food safety.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序