Recombinant Porcine Interferon-α Decreases Pseudorabies Virus Infection

文献类型： 外文期刊

第一作者： Song, Bowen

作者： Song, Bowen;Liu, Xueyi;Huang, Yaoyao;Zhu, Shuaiqi;Yi, Lin;Ding, Hongxing;Zhao, Mingqiu;Chen, Jinding;Wei, Wenkang;Zhao, Mingqiu;Eerdunfu, Mingqiu

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关键词： interferon-alpha; pseudorabies virus; mammalian expression system; CHO-K1 cell

期刊名称：VACCINES （ 影响因子：7.8； 五年影响因子：7.4 ）

ISSN：

年卷期： 2023 年 11 卷 10 期

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收录情况： SCI

摘要： Interferon (IFN) is a cell-secreted cytokine possessing biological activities including antiviral functioning, immune regulation, and others. Interferon-alpha (IFN-alpha) mainly derives from plasmacytoid dendritic cells, which activate natural killer cells and regulate immune responses. IFN-alpha responds to the primary antiviral mechanism in the innate immune system, which can effectively cure acute infectious diseases. Pseudorabies (PR) is an acute infectious disease caused by pseudorabies virus (PRV). The clinical symptoms of PRV are as follows: reproductive dysfunction among pregnant sows and high mortality rates among piglets. These pose a severe threat to the swine industry. Related studies show that IFN-alpha has broad applications in preventing and treating viral diseases. Therefore, a PRV mouse model using artificial infection was established in this study to explore the pathogenic effect of IFN-alpha on PRV. We designed a sequence with IFN-alpha 4 (M28623, Genbank) and cloned it on the lentiviral vector. CHO-K1 cells were infected and identified using WB and RT-PCR; a CHO-K1 cell line with a stable expression of the recombinant protein PoIFN-alpha was successfully constructed. H&E staining and virus titer detection were used to investigate the recombinant protein PoIFN-alpha's effect on PR in BALB/c mice. The results show that the PoIFN-alpha has a preventive and therapeutic impact on PR. In conclusion, the recombinant protein can alleviate symptoms and reduce the replication of PRV in vivo.

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