Multi-omics insights into the regulatory mechanism of citric acid in silage fermentation

文献类型： 外文期刊

第一作者： Ma, Jingbo

作者： Ma, Jingbo;Ma, Yingying;Liang, Runfei;Yang, Jiarui;Yao, Junhu;Li, Zongjun;Zi, Xuejuan;Wu, Shuo;Li, Mao

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关键词： Citric acid; Whole-plant cassava silage; Meta-analysis; Metagenome; Metabolome

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.0； 五年影响因子：9.5 ）

ISSN： 0960-8524

年卷期： 2025 年 436 卷

页码：

收录情况： SCI

摘要： A meta-analysis was conducted to assess the effects of citric acid (CA) on silage fermentation, and then used whole-plant cassava silage as a model to explore the underlying microbiological mechanisms with metagenomic and metabolomic data. The meta-analysis revealed that CA supplementation increased the dry matter, crude protein, water-soluble carbohydrate, and lactic acid contents in silage, but decreased the pH, dry matter loss, and the contents of fiber, NH3-N, and acetic acid, all of which meet the expectations for an ideal silage additive. The fermentation parameter responses of whole-plant cassava silage to CA were consistent with those in the metaanalysis. Metabolomic analysis revealed that CA increased the level of antimicrobial metabolites and decreased the level of amino acids and their derivatives in cassava silage. By constructing microbial genome and gene catalogs, we found that CA supplementation increased the abundance of lactic acid-rods (Levilactobacillus, Lentilactobacillus, and Companillactobacillus) and inhibited the abundance of lactic acid cocci (Leuconostoc, Pediococcus, and Weissella) and undesirable bacteria (Acinetobacter, Serratia, Klebsiella, and Pantoea), which resulted in an increased abundance of genes involved in structural carbohydrate hydrolysis (cellulase and pectinase), lactic acid production (ldh), and amino acid synthesis (CKase and CPS1) and a decreased abundance of genes

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