Marker assisted detection of TYLCV and late blight resistance in tomato (Solanum lycopersicum)

文献类型： 外文期刊

第一作者： Mangal, Manisha

作者： Mangal, Manisha;Hussain, Zakir;Lata, Suman;Gosavi, Gokul;Tomar, B. S.;Gosavi, Gokul

作者机构：

关键词： Late blight; MAS; SCAR; Tomato; Tomato leaf curl disease

期刊名称：INDIAN JOURNAL OF AGRICULTURAL SCIENCES （ 影响因子：0.374； 五年影响因子：0.447 ）

ISSN： 0019-5022

年卷期： 2021 年 91 卷 10 期

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收录情况： SCI

摘要： Marker assisted breeding relies on the identification of genetic loci linked to desirable genes/alleles and is being utilized for selecting desirable traits including disease resistance in a wide variety of crops. The tomato (Solanum lycopersicum L.) production worldwide has lately been threatened by outbreaks of tomato yellow leaf curl virus transmitted by the whitefly (Bemisia tahaci) and late blight caused by oomycete pathogen Phytophthora infestans (Mont) de Bary. Resistance genes against both pathogens are available and widely used in tomato breeding throughout the world. Molecular markers for resistance alleles greatly enhance selection of resistant plants. For effective breeding programme, the validation of markers linked to resistance genes in tomato was carried out for tomato improvement. For this purpose, available markers for the tomato yellow leaf curl disease resistance gene loci Tv-2, Ty3, Ty3a and late blight resistant loci Ph3 were validated The assay correctly predicted the genotypes of tomato breeding lines harbouring resistance allele at each loci. Further a multiplex assay was also standardized to simultaneously detect the presence of resistant/susceptible alleles at Ty2, Ty3 and Ty3a loci. The present study carried out in the Division of Vegetable Science, ICAR-IARI, New Delhi, demonstrates the feasibility of combining two SCAR markers (TG0302 and P6-25) for detecting simultaneously three important resistance gene loci in tomato genotypes and thereby improving the cost and efficiency of the assay.

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