Isolation, characterization and comparison of lytic Epseptimavirus phages targeting Salmonella

文献类型： 外文期刊

第一作者： Cong, Cong

作者： Cong, Cong;Cui, HuiJing;Li, XiaoYu;Yuan, YuYu;Wang, LiLi;Xu, YongPing;Wei, BingDong;Li, ShuYing;Xu, YongPing;Wei, BingDong;Li, XiaoYu;Wang, LiLi;Xu, YongPing

作者机构：

关键词： Phage; Genus Epseptimavirus; Salmonella; Genomic comparison; Taxonomy; Food control; Foodborne pathogen

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：6.475； 五年影响因子：6.508 ）

ISSN： 0963-9969

年卷期： 2021 年 147 卷

页码：

收录情况： SCI

摘要： This study describes the characterization and genomic analysis of six lytic Salmonella phages. To examine the feasibility of using these phages as biocontrol agents, we analyzed their genomes and compared them to those of similar phages. These six phages belong to genus Epseptimavirus, family Demerecviridae. We identified the genes of these six phages by comparing their genomes with those of three type phages in subfamily Markadamsvirinae. All six phages examined in this study were obligately lytic and did not carry undesirable genes. Two phages (vB_SalS_1-23 and vB_SalS_3-29) were selected as the representative phages for general characterization and physiological tests. The biocontrol efficacy of the representative phages was determined by comparing the viable counts of recovered host Salmonella ser. Newlands ZC-S1 from treatment and phage-free control samples. The biocontrol experiment showed that the representative phages were able to reduce the counts of ZC-S1 to below 2 log(10) CFU/mL (similar to 4.3 log(10) CFU/mL reduction) at 3 h post-infection at 37 degrees C. Furthermore, we investigated the application of these two phages in the control of ZC-S1 contamination in chicken products and on eggshells. When applied to the surfaces of the samples, the phage cocktail (MOI = 100) reduced the ZC-S1 count to below 2 log(10) CFU/mL on chicken skin and to undetectable levels (1 log(10) CFU/mL) in chicken breast meat, ground chicken meat and eggshell samples (p < 0.01). Compared to the initial experiment, the phage cocktail reduced the ZC-S1 count by 2-4.08 log(10) CFU/mL when applied at an MOI = 1 (except in the ground chicken meat group) and by 4.48-5.67 log(10) CFU/mL at an MOI = 100 after 7 h. In conclusion, these two phages with lytic effects show a high potential to inhibit the growth of Salmonella contaminants and can be used as candidate biocontrol agents.

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