An integrated approach to improve the assay performance of quantum dot-based lateral flow immunoassays by using silver deposition

文献类型： 外文期刊

第一作者： Wang, Yulong

作者： Wang, Yulong;Liu, Pengyan;Ye, Yuhui;Zhang, Cunzheng;Wang, Yulong;Wang, Yulong;Zhang, Cunzheng;Hammock, Bruce D.;Zhang, Cunzheng

作者机构：

关键词： Quantum dot; Lateral flow immunoassay; Silver deposition; Sodium pentachlorophenate; Signal enhancement

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：4.8； 五年影响因子：4.5 ）

ISSN： 0026-265X

年卷期： 2023 年 192 卷

页码：

收录情况： SCI

摘要： Traditional quantum dot-based lateral flow immunoassay (QD-LFIA) is limited to signal loss in part by the blinking, photobleaching and oxidative quenching of QD probes. Inspired by the good application of silver deposition on QD surfaces in tissue imaging, and in the context of improving the assay performance without compromising the simplicity and practicality, we report that introducing the QD-silver combination to the LFIA system, has the advantages of accuracy improvement, signal enhancement and user friendliness promotion, but maintains the cost-effective property and commercial accessibility of QD-LFIA. The effect was shown by using CdSe/ZnS QD-LFIA coupled with anti-sodium pentachlorophenate antibody, which provided a 4-fold improvement in the signal, a 2.5-fold improvement in the detection limit and a zero false-negative rate for sodium pentachlorophenate analysis in chicken samples. The proposed LFIA integrates the possibilities of colorimetric and fluorometric detection with different detection limits (fluorometric at 10 ng/mL and colorimetric at 4 ng/ mL) and with acceptable detection times (fluorometric at 12 min and colorimetric at 27 min). The current results indicate that this QD-silver combined LFIA is complementary to conventional fluorescence LFIA and could be an inexpensive, versatile, and sensitive alternative.

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