Screening of hypoglycemic and probiotic properties of Pediococcus acidilactici FM-Pa-JXM94 and exploring the potential hypoglycemic mechanism

文献类型： 外文期刊

第一作者： Wang, Ying

作者： Wang, Ying;Zhang, Hui;Wang, Xingna;Zhang, Yu;Fan, Linlin;Ma, Yanhong;Liu, Xiaoli;Wang, Ying;Zhang, Hui;Liu, Xiaoli;Wang, Ying

作者机构：

关键词： Pediococcus acidilactici; alpha-Amylase and alpha-glucosidase inhibition; activities; Lactic acid bacteria; Probiotic properties; Hypoglycemic function

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.9； 五年影响因子：6.1 ）

ISSN： 2212-4292

年卷期： 2025 年 68 卷

页码：

收录情况： SCI

摘要： In this study, alpha-amylase and alpha-glucosidase inhibition activities of cell-free supernatant (CFS) of 216 lactic acid bacteria were measured to screen lactic acid bacteria resources with potential hypoglycemic functions. Significant inhibition activities were detected in Pediococcus acidilactici FM-Pa-JXM94, L15, GC215 and L31, and their probiotic properties were further assessed. Following the inhibitory activities on alpha-glucosidase and alpha-amylase, the viability of simulated gastric juice and adhesive ability, FM-Pa-JXM94 was screened and identified as Pediococcus acidilactici by 16S rDNA nucleotide sequencing, and the potential hypoglycemic mechanism was explored using IR-HepG2. CFS, cell-free extract (CFE) and bacterial cells of FM-Pa-JXM94 demonstrated a strong potential to induce glucose consumption, glycogen synthesis, and pyruvate kinase activity, as well as decrease phosphoenolpyruvate carboxykinase activity of IR-HepG2 cells. Additionally, RT-qPCR results showed that CFS, CFE and bacteria cells upregulated the mRNA expression of IRS1, AKT, GYS2 and PI3K genes and downregulated the mRNA expression of GSK3(3, FOXO1, PEPCK and G-6-Pase genes of IRS-1/PI3K/AKT/GSK3(3/GYS2 and PI3K/ AKT/FOXO1/PEPCK-G6Pase signal pathways. These findings illustrate that the different FM-Pa-JXM94 derivatives could alleviate insulin resistance of IR-HepG2 cells from key enzyme activity and gene levels. This study showcases FM-Pa-JXM94 as a promising probiotic candidate for preparing functional foods and hypoglycemic function supplements.

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