Virome Analysis for Identification of a Novel Porcine Sapelovirus Isolated in Western China

文献类型： 外文期刊

第一作者： Chen, Jianing

作者： Chen, Jianing;Suo, Xuepeng;Cao, Liyan;Yuan, Cong;Shi, Lei;Duan, Yueyue;Zheng, Haixue;Wang, Qi;Suo, Xuepeng;Cao, Liyan;Yuan, Cong;Shi, Lei;Duan, Yueyue;Wang, Qi;Suo, Xuepeng;Cao, Liyan;Yuan, Cong;Shi, Lei;Duan, Yueyue;Wang, Qi

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关键词： biological characterization; diarrhea; next-generation sequencing; porcine sapelovirus

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：9.043； 五年影响因子：8.113 ）

ISSN： 2165-0497

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收录情况： SCI

摘要： Diarrhea is one of the most important problems associated with the production of piglets, which have a wide range of possible pathogens. This study identified a strain of porcine sapelovirus (PSV) by using next-generation sequencing (NGS) technologies as the pathogen among fecal samples in a pig herd. Phylogenetic analysis showed that the PSV isolates shared a unique polyprotein and clustered with Chinese isolates identified before 2013. The PSV strain was then isolated and named GS01. The in vitro and in vivo biological characteristics of this virus were then described. Our pathogenicity investigation showed that GS01 could cause an inflammatory reaction and induce serious diarrhea in neonatal piglets. To our knowledge, this is the first isolation and characterization of PSV in western China. Our results demonstrate that the PSV GS01 strain is destructive to neonatal piglets and might show an expanded role for sapeloviruses. IMPORTANCE Porcine sapelovirus (PSV) infection leads to severe polioencephalomyelitis with high morbidity and mortality, resulting in significant economic losses. In previous studies, PSV infections were always subclinical or only involved a series of mild symptoms, including spinal cord damage, inappetence, diarrhea, and breathless. However, in our study, we isolated a novel PSV by virome analysis. We also determined the biological characteristics of this virus in vitro and in vivo. Our study showed that this novel PSV could cause an inflammatory response and induce serious diarrhea in neonatal piglets. To our knowledge, this is the first isolation and characterization of PSV in western China. These findings highlight the importance of prevention for the potential threats of PSV. Porcine sapelovirus (PSV) infection leads to severe polioencephalomyelitis with high morbidity and mortality, resulting in significant economic losses. In previous studies, PSV infections were always subclinical or only involved a series of mild symptoms, including spinal cord damage, inappetence, diarrhea, and breathless.

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[1]Virome Analysis for Identification of a Novel Porcine Sapelovirus Isolated in Western China. Suo, Xuepeng,Cao, Liyan,Yuan, Cong,Shi, Lei,Duan, Yueyue,Wang, Qi,Chen, Jianing,Suo, Xuepeng,Cao, Liyan,Yuan, Cong,Shi, Lei,Duan, Yueyue,Zheng, Haixue,Wang, Qi,Suo, Xuepeng,Cao, Liyan,Yuan, Cong,Shi, Lei,Duan, Yueyue,Wang, Qi. 2022

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