The antibacterial activity of a novel NK-lysin homolog and its molecular characterization and expression in the striped catfish, Pangasianodon hypophthalmus

文献类型： 外文期刊

第一作者： Ruan, Zhuo-Hao

作者： Ruan, Zhuo-Hao;Li, Yi-Fu;Jiang, Liang-Sen;Lu, Zhi-Qiang;Luo, Yuan-Yuan;Liu, Wen-Sheng;Ruan, Zhuo-Hao;Huang, Wen;Li, Yi-Fu;Jiang, Liang-Sen;Lu, Zhi-Qiang;Luo, Yuan-Yuan;Liu, Wen-Sheng;Ruan, Zhuo-Hao;Liu, Wen-Sheng;Zhang, Xi-Quan

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关键词： Pangasianodon hypophthalmus; Aeromonas hydrophila; Antimicrobial peptides; NK-Lysin; Antibacterial activity

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）

ISSN： 1050-4648

年卷期： 2022 年 127 卷

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收录情况： SCI

摘要： Aeromonas hydrophila was a common bacterial pathogen in aquaculture resulting in considerable losses to the striped catfish aquaculture industry. As an emergent antimicrobial peptide (AMP), NK-lysin (NKL) had activity against various microorganisms. However, the antibacterial activity of NKL from striped catfish (Pangasianodon hypophthalmus) both in vitro and vivo remains unclear. In this study, the cDNA sequence of P. hypophthalmus NK-lysin gene (PhNK-lysin) was cloned and characterized. The amino acid sequence of PhNK-lysin contains a signal peptide sequence of 17 amino acid (aa) residues and a mature peptide composed of 130 aa. The saposin B domain of mature peptide comprised six conserved cysteines forming three putative disulfide bonds. Phylogenetic analysis revealed that the PhNK-lysin was most closely related to that of the channel catfish (Ictalurus punctatus) NK-lysin. The transcriptional levels of the PhNK-lysin were significantly upregulated in response to A. hydrophila infection in various tissues including heart, liver, spleen, head kidney, trunk kidney and gill. The synthetic PhNK-lysin-derived peptide consisting of 38aa showed antibacterial activity against Vibrio harveii, Aeromonas hydrophila and Escherichia coli. The MIC for V. harveii, A. hydrophila and E. coli were 15.625 mu M, 250 mu M and 31.25 mu M respectively. Besides, the synthetic PhNK-lysin decreased the bacterial load of liver and trunk kidney in vivo as well as increased the survival rate of A. hydrophila infected striped catfish. Hence, these data suggest that PhNK-lysin had antimicrobial effect and protects the host from pathogenic infection.

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