Different contributions of rare microbes to driving soil nitrogen cycles in acidic soils under manure fertilization

文献类型： 外文期刊

第一作者： Wang, Chao

作者： Wang, Chao;Guo, Long;Shen, Ren Fang;Wang, Chao;Cai, Ze Jiang;Chen, Juan;Wang, Chao;Guo, Long;Shen, Ren Fang

作者机构：

关键词： Acidic soils; Denitrifies; Diazotrophs; Nitrifiers; Organic manure

期刊名称：APPLIED SOIL ECOLOGY （ 影响因子：4.8； 五年影响因子：5.1 ）

ISSN： 0929-1393

年卷期： 2024 年 196 卷

页码：

收录情况： SCI

摘要： Organic manure can substantially improve soil fertility and influence soil microbes. Despite the pivotal role of Ncycling microbes in agro-ecosystems, little is known about the importance of rare microbes to N cycles in acidic soils under manure fertilization. Here, we conducted a 11 -year field experiment in which chemical N fertilizers were substituted with swine manure at different doses in an acidic soil. We investigated soil biological N fixation (BNF), potential nitrification rate (PNR), denitrifying enzyme activity (DEA), and associated microbial communities (diazotrophs, ammonia oxidizing bacteria (AOB), ammonia oxidizing archaea (AOA), and nirK- and nirS-type denitrifiers). With the increase of manure dose, BNF, PNR, DEA and abundances of soil N-cycling functional genes significantly increased. Manure application changed whole and subgroup (abundant, intermediate, and rare taxa) community structures of these N-cycling microbes and increased Shannon indexes of AOA, AOB and nirK-type denitrifiers but not diazotrophs and nirS-type denitrifiers. The community structures and Shannon variations of rare AOA and AOB subgroups highly contributed to PNR and subgroup community structures of rare nirK- and nirS-type denitrifiers were significantly related to DEA, but rare diazotrophs did not contribute to BNF. In the co-occurrence networks of AOA, AOB, nirK- and nirS-type denitrifiers, most keystone species were rare taxa, and the positive edge numbers belonging to rare taxa were positively related to PNR and DEA. Manure-derived microbial species provided the very low contributions to community dissimilarities of these soil N-cycling microbes. Our findings suggested that rare microbes made surprisingly important contributions to soil nitrification and denitrification rather than N-fixation in manured acidic soils.

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