Molecular cloning and tissue transcriptional analysis of a novel gene encoding proteasome activator PA28-alpha from common carp (Cyprinus carpio L.)

文献类型： 外文期刊

第一作者： Tan, Yeping

作者： Tan, Yeping;Lu, Qiang;Li, Lianrui;Zhu, Yanlei;Tan, Yeping;Fu, Baoquan

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关键词： cDNA library;gene organization;sequence divergence

期刊名称：FISHERIES SCIENCE （ 影响因子：1.617； 五年影响因子：1.531 ）

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收录情况： SCI

摘要： To identify the homolog of the PA28-alpha subunit, a leukocyte cDNA library of common carp was screened and the full-length cDNA sequence of the PSME1 gene coding for the PA28-alpha was obtained. It encompasses 1097 nucleotides (nt), with an open reading frame encoding 249 amino acids. The deduced protein sequence exhibits identities of 92, 78, 78, 77, 75, 56, 54, 53 and 54% with zebrafish, Atlantic salmon, northern pike, rainbow trout, rainbow smelt, mouse, pig, cattle and human PA28-alpha subunits, respectively. Multiple sequence alignment of the PA28-alpha protein of common carp and other known PA28 subunits indicates that it contains a PA28-alpha subunit-specific insert corresponding to the KEKE motif of the known PA28-alpha (Region B), a characteristic proline-rich motif (Region A), a potential protein kinase C recognition site (Region D), a conserved activation loop (Region C), and a highly homologous C-terminal region (Region E). Phylogenetic analysis reveals that the PA28-gamma subunit is related to the presumed ancestor of the PA28-alpha and PA28-beta subunits. In addition, the genomic DNA obtained by PCR is composed of eleven exons and ten introns with 3583 nt, typical of the PA28-alpha gene organization. Compared with the other known PA28-alpha genomic DNA, in spite of structural conservation through evolution, the fish PA28-alpha genes showed a much greater sequence divergence than their counterparts among mammals. Tissue transcriptional analysis of carp PA28-alpha indicated that it was visibly enhanced in tissues associated with immunity (gill, spleen, kidney and liver), and weakly transcribed in muscle, brain and heart.

分类号： S9

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