文献类型： 外文期刊
第一作者： Chen, Guohua
作者： Chen, Guohua;He, Xiaobing;Gao, Zhenzhen;Fang, Yongxiang;Hurisa, Takele Tesgera;Jia, Huaijie;Tan, Jinlong;Zhou, Guangqin;Fu, Baoquan;Li, Weike;Jing, Zhizhong;Chen, Guohua;He, Xiaobing;Gao, Zhenzhen;Fang, Yongxiang;Hurisa, Takele Tesgera;Jia, Huaijie;Tan, Jinlong;Zhou, Guangqin;Fu, Baoquan;Li, Weike;Jing, Zhizhong;Chen, Guohua;He, Xiaobing;Gao, Zhenzhen;Fang, Yongxiang;Hurisa, Takele Tesgera;Jia, Huaijie;Tan, Jinlong;Zhou, Guangqin;Fu, Baoquan;Li, Weike;Jing, Zhizhong;Chen, Guohua;He, Xiaobing;Gao, Zhenzhen;Fang, Yongxiang;Hurisa, Takele Tesgera;Jia, Huaijie;Tan, Jinlong;Zhou, Guangqin;Fu, Baoquan;Li, Weike;Jing, Zhizhong
作者机构：
关键词： Competitive ELISA; LSDV; A33; Polyclonal antibody; Serological investigation
期刊名称：VIROLOGY JOURNAL （ 影响因子：4.0； 五年影响因子：3.8 ）
ISSN：
年卷期： 2024 年 21 卷 1 期
页码：
收录情况： SCI
摘要： Goatpoxvirus (GTPV), sheeppoxvius (SPPV), and the Lumpy skin disease virus (LSDV) is a Capripoxvirus belonging to the family poxviridae. They can cause significant economic losses in countries where this disease are endemic. However, effective and convenient diagnostic tools against sera antibody are not readily available until now. Toward this goal, a polyclonal antibody competitive enzyme-linked immunosorbent assay (c-ELISA) of detecting serogroup-specific antibody is established based on major LSDV antigen A33. Serum samples (n = 605) were collected to optimize the c-ELISA from different areas. The cut-off value for the c-ELISA was estimate using percent inhibition (PI) values. The diagnostic performance of test including sensitivity (sn) and specificity (sp) were obtained by receiver operator characteristic (ROC) analysis. Among these analysis, > 57.61% PI value was accepted as cut-off of the c-ELISA, the diagnostic sn an diagnostic sp were reached to 96.4% and 98.5%, at > 95% confidence interval. These results show that the developed competitive ELISA is sensitive, specific, and reliable, which make it appropriate for serological investigation.
分类号：
- 相关文献
作者其他论文 更多>>
-
Global profiling of protein S-palmitoylation in the second-generation merozoites of Eimeria tenella
作者：Qu, Zigang;Li, Wenhui;Zhang, Nianzhang;Olajide, Joshua Seun;Fu, Baoquan;Qu, Zigang;Li, Wenhui;Zhang, Nianzhang;Olajide, Joshua Seun;Fu, Baoquan;Qu, Zigang;Li, Wenhui;Zhang, Nianzhang;Olajide, Joshua Seun;Fu, Baoquan;Li, Yuqiong;Mi, Xiaoyun;Qu, Zigang;Li, Wenhui;Zhang, Nianzhang;Fu, Baoquan
关键词：Protein acylation; E. tenella; Bioiological process; The second-generation merozoites
-
Parasite-derived microRNA let-7-5p detection for metacestodiasis based on rolling circular amplification-assisted CRISPR/Cas9
作者：Wang, Liqun;Pu, Guiting;Liu, Tingli;Chen, Guoliang;Li, Hong;Amuda, Tharheer Oluwashola;Cao, Shanling;Yan, Hongbin;Yin, Hong;Fu, Baoquan;Luo, Xuenong;Yin, Hong;Fu, Baoquan;Luo, Xuenong;Fu, Baoquan;Luo, Xuenong
关键词：diagnosis; let-7-5p; metacestodiasis; plasma; RCA-assisted CRISPR/Cas9
-
Parasite-derived microRNA let-7-5p detection for metacestodiasis based on rolling circular amplification-assisted CRISPR/Cas9
作者：Wang, Liqun;Pu, Guiting;Liu, Tingli;Chen, Guoliang;Li, Hong;Amuda, Tharheer Oluwashola;Cao, Shanling;Yan, Hongbin;Yin, Hong;Fu, Baoquan;Luo, Xuenong;Yin, Hong;Fu, Baoquan;Luo, Xuenong;Fu, Baoquan;Luo, Xuenong
关键词：diagnosis; let-7-5p; metacestodiasis; plasma; RCA-assisted CRISPR/Cas9
-
Transcriptome analysis of long non-coding RNA and mRNA Profiles in VSV-infected BHK-21 Cells
作者：Han, Wuweiyi;Liu, Yongsheng;Han, Wuweiyi;Yang, Fan;Sun, Xintong;Yang, Jianshe;Qiu, Jinxin;Zhang, Luhua;Zhang, Wenhui;Chen, Guohua;Li, Weike;Fei, Xiaojuan;Han, Wei;He, Xiaobo
关键词：Vesicular stomatitis virus; RNA-Seq; LncRNA; mRNA; Differentially expressed genes
-
Alterations of the gut microbiota in type 2 diabetics with or without subclinical hypothyroidism
作者：Lv, Yanrong;Liu, Rong;Sun, Xiaolan;Gong, Yuhan;Ma, Li;Wang, Xiaoxia;Jia, Huaijie;Qiu, Wei
关键词：Type 2 Diabetes Mellitus; Subclinical hypothyroidism; Gut microbiota; DNA copy number variation; Salivary alpha-amylase gene
-
Effects of different host plants on the growth, development, and fecundity of Phthorimaea absoluta (Lepidoptera: Gelechiidae): an evaluation based on the age-stage two-sex life table
作者：Jiang, Zhengxiong;Yang, Guiqun;Zhang, Jinlong;Chen, Guohua;Chen, Heng;Zhang, Xiaoming;Yang, Guiqun;Hu, Changxiong;Zhang, Xiaoming;Zhang, Xiaoming
关键词：P; absoluta; L; esculentum; S; tuberosum; melongena; N; tabacum
-
Lumpy Skin Disease Virus Infection Activates Autophagy and Endoplasmic Reticulum Stress-Related Cell Apoptosis in Primary Bovine Embryonic Fibroblast Cells
作者：Tan, Jinlong;Liu, Yinju;Li, Weike;Zhang, Yongzhi;Chen, Guohua;Fang, Yongxiang;He, Xiaobing;Jing, Zhizhong
关键词：lumpy skin disease virus; autophagy; endoplasmic reticulum; apoptosis
