The efficacy of FMD vaccine reduced non-structural proteins with a mAb against 3B protein

文献类型： 外文期刊

第一作者： Li, Dong

作者： Li, Dong;Liu, Zai-Xin;Sun, Pu;Li, Yong-Liang;Lu, Zeng-Jun;Tian, Mei-Na;Chen, Ying-Li;Xie, Bao-Xia;Bao, Hui-Fang;Fu, Yuan-Fang;Cao, Yi-Mei;Li, Ping-Hua;Bai, Xin-Wen;Sun, Jia-Chuan;Guo, Jian-Hong;Liu, Xiang-Tao;Xie, Qing-Ge

作者机构：

关键词： Biochemistry and Molecular Biophysics;Pharmacology;Infection;Immune System: Chemical Coordination and Homeostasis;Veterinary Medicine: Medical Sciences

期刊名称：VETERINARY RESEARCH COMMUNICATIONS （ 影响因子：2.459； 五年影响因子：2.352 ）

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年卷期：

页码：

收录情况： SCI

摘要： A monoclonal antibody, 3BIgG, against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B was obtained. The 3BIgG-sepharose conjugant (3BmAb-6BFF) was prepared by adding the purified 3BIgG into epoxy-activated sepharose 6BFF, incubating with the inactivated FMDV, and then removing the sepharose by centrifugation. The vaccine was made from the supernatant emulsified with oil-adjuvant ISA206. Ten guinea pigs, 26 pigs and six cattle were vaccinated, and a vaccination control group was included without treatment with 3BmAb-6BFF. After 28 days, 9/10 pigs challenged with FMDV were protected, this result was the same as the control group, indicating that the vaccine potency was not reduced after treatment with 3BmAb-6BFF. The other animals were vaccinated weekly for nine weeks, and serum samples were collected to detect 3ABC-antibody titers. The results showed that 3ABC-antibody production was delayed and the positive antibody rates were lower when vaccination was carried out using vaccines treated with 3BmAb-6BFF compared with untreated vaccines. The findings of this study suggest that it is possible to reduce NSPs using a mAb-sepharose conjugant in FMD vaccines without reducing their efficacy.

分类号： S85

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