Multiplex Approach of Metabolomic and Transcriptomic Reveals the Biosynthetic Mechanism of Light-induced Flavonoids and CGA in Chrysanthemum

文献类型： 外文期刊

第一作者： Lu, Chenfei

作者： Lu, Chenfei;Liu, Yuchen;Yan, Xiaoyun;Shao, Qingsong;Lu, Chenfei;Liu, Yuchen;Yan, Xiaoyun;Gui, Aijun;Jiang, Yilu;Shao, Qingsong;Wang, Pan;Qiao, Qian

作者机构：

关键词： Chrysanthemum morifolium; Light induction; Flavonoids; CGA; Transcriptional regulation

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.6； 五年影响因子：5.7 ）

ISSN： 0926-6690

年卷期： 2024 年 221 卷

页码：

收录情况： SCI

摘要： Light, as an important environmental signal for plant growth and development, has been reported to be involved in the metabolism of phenylpropane. However, the light-induced phenylpropane biosynthesis mechanism in Chrysanthemum morifolium Ramat., especially flavonoid and chlorogenic acid (CGA) metabolism, is not clear. In this study, we found the flower phenotype of chrysanthemum 'HangBaiJu' became lighter after bagging. Besides, the content of main active ingredients, such as Luteolin-7-O-glucoside (Lu-7-O-G), Diosmetin-7-O-glucoside (Di- 7-O-G), Apigenin-7-O-glucoside, Apigenin and CGA, was significantly decreased. Comparative transcriptome analysis (pairwise comparison, WGCNA, and k-means clustering) revealed that the expression of structural genes, such as CmPAL, , CmCHS1/2, CmF3'H, CmFNS and CmHQT, was notably declined under dark conditions. Meanwhile, 'bridge proteins' CmMYB3/6/16 and signal transduction factors CmBBX20/22.2/22.3 were identified as key regulatory factors in the light-mediated synthesis of flavonoids and CGA in chrysanthemum. Among that, CmBBX20 could promote the accumulation of flavonoids and CGA by directly binding to the G-box core sequences of downstream target genes based on transient overexpression and Y1H assays. Overall, the preliminary molecular mechanism of BBXs and MYBs coordinately regulating the accumulation of flavonoids and CGA in chrysanthemum under different light inductions has been clarified, which provided a theoretical basis for the molecular breeding and quality improvement of chrysanthemum.

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