Molecular characterization and expression analysis of chitinase from the pearl oyster Pinctada fucata

文献类型： 外文期刊

第一作者： Li, Haimei

作者： Li, Haimei;Wang, Deqing;Deng, Zhenghua;Huang, Guiju;Fan, Sigang;Zhou, Daizhi;Liu, Baosuo;Zhang, Bo;Yu, Dahui;Li, Haimei;Wang, Deqing;Deng, Zhenghua;Huang, Guiju;Fan, Sigang;Zhou, Daizhi;Liu, Baosuo;Zhang, Bo;Yu, Dahui;Li, Haimei;Wang, Deqing;Deng, Zhenghua;Zhou, Daizhi

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关键词： Chitinase-1 gene; Expression profile; Shell formation; Biomineralization; In situ hybridization; Larval stage; Pinctada fucata

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

ISSN： 1096-4959

年卷期： 2017 年 203 卷

页码：

收录情况： SCI

摘要： Chitinase is an enzyme that plays an important role in the chitin metabolism of a wide range of organisms. However, the function of chitinase in the pearl oyster Pinctada fucata is yet to be determined. In this study, a chitinase gene (named PfChi1) was cloned from P. fucata and its expression profiles were investigated. The full-length cDNA of PfChi1 was 2743 bp and consisted of a 2187-bp open reading frame encoding 728 amino acid residues, a 47-bp 5'-untranslated region (UTR), and a 509-bp 3'-UTR. Similar to other known chitinases, the PfChil protein is composed of an N-terminal leading signal peptide, a catalytic domain, a linker region, and a C-terminal chitin binding domain. The results of qRT-PCR showed that PfChil was expressed in a wide range of tissues with relatively high levels in the mantle, muscle, gill, and gonad, and relatively low levels in hemocytes, the intestine, and the digestive gland (P < 0.05). In situ hybridization showed that PfChil was mainly expressed in the mantle edge, particularly in the outer epithelial cells of the inner fold, whereas few hybridization signals were detected in the inner epithelial cells of the middle fold. A shell damage experiment indicated that PfChil transcript levels were up-regulated significantly (P< 0.05) at 24 h after shell damage and decreased gradually thereafter, followed by shell regeneration, indicating that PfChil is involved in shell formation. In addition, PfChil expression was higher in trochophore larvae than in other developmental stages (P < 0.05), indicating a possible association with the formation of prodissoconch shells. To the best of our knowledge, this study is the first to report the potential biomineralization function of a chitinase in P. fucata. (C) 2016 Elsevier Inc. All rights reserved.

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