Glutamine and glutaminolysis are required for efficient replication of infectious spleen and kidney necrosis virus in Chinese perch brain cells

文献类型： 外文期刊

第一作者： Fu, Xiaozhe

作者： Fu, Xiaozhe;Li, Ningqiu;Lin, Qiang;Huang, Zhibin;Fu, Xiaozhe;Hu, Xianqin;Li, Ningqiu;Zheng, Feifei;Dong, Xingxing;Duan, Jing;Lin, Qiang;Tu, Jiagang;Zhao, Lijuan;Su, Jianguo;Lin, Li;Hu, Xianqin;Fu, Xiaozhe;Su, Jianguo;Lin, Li

作者机构：

关键词： siniperca chuatsi; ISKNV; glutamine; glutaminolysis; TCA cycle

期刊名称：ONCOTARGET （ 影响因子：5.168； 五年影响因子：5.312 ）

ISSN： 1949-2553

年卷期： 2017 年 8 卷 2 期

页码：

收录情况： SCI

摘要： Viruses rely on host cellular metabolism for energy and macromolecule synthesis during their replication. Infectious spleen and kidney necrosis virus (ISKNV) causes significant economic losses in the Chinese perch (Siniperca chuatsi) industry worldwide. However, little is known about the relationship between ISKNV replication and cellular metabolism. Using transcriptomic analysis, we observed that glutamine metabolism in Chinese perch brain (CPB) cells is altered during ISKNV infection. Moreover, ISKNV replication was decreased in CPB cells cultured in the glutamine-depleted medium. ISKNV replication was also inhibited in CPB cells cultured in the presence of bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl) ethyl sulfide (an inhibitor of glutaminase), (-)-epigallocatechinmo nogallate (an inhibitor of glutamate dehydrogenase) or L-buthionine sulfoximine (an inhibitor of glutathione synthesis). However, virus replication was rescued by the addition of multiple tricarboxylic acid cycle intermediates, ATP, or glutathione reduced ethyl ester. ATP and reduced glutathione/oxidized glutathione levels were increased in CPB cells infected with ISKNV, but were decreased in CPB cells cultured in glutamine-depleted medium. These results indicate ISKNV infection induces glutaminolysis to accommodate the biosynthetic and energy needs for its efficient virus replication.

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