The immune response of the C-Jun in the black tiger shrimp (Penaeus monodon) after bacterial infection

文献类型： 外文期刊

第一作者： Shi, Gongfang

作者： Shi, Gongfang;Zhao, Chao;Fu, Mingjun;Qiu, Lihua;Shi, Gongfang;Shi, Gongfang;Zhao, Chao;Fu, Mingjun;Qiu, Lihua;Qiu, Lihua

作者机构：

关键词： Penaeus monodon; C-Jun; Vibro harveyi; Streptococus agalactiae; qRT-PCR; In situ hybridization

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2017 年 61 卷

页码：

收录情况： SCI

摘要： The transcription factor C-Jun widely exists in vertebrates and invertebrates and plays an important role in various kinds of stimulus response. In this study, PmC-Jun gene was first cloned from Penaeus monodon. The full-length cDNA of PmC-jun was 1857 bp in length and included an 879 bp open reading frame (ORF), which encoded 293 amino acids. qRT-PCR analysis results showed that PmC-jun mRNAs were ubiquitously expressed in all the examined tissues. The highest expression level was observed in gill, followed by hepatopancreas. The expression patterns of PmC-jun after Vibrio harveyi and Streptococcus agalactiae injections were studied by qRT-PCR experiment. PmC-jun increased obviously in the gill and hepatopancreas. The expression pattern of PmC-jun in the hepatopancreas was further studied using in situ hybridization (ISH) method. The mRNA expression level of PmC-jun significantly increased in the hepatopancreas after bacterial infection. The expression sites of PmC-jun were almost unchanged. PmC-jun played a regulatory role in pathogen invasion. (C) 2016 Elsevier Ltd. All rights reserved.

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