The effects of emodin on cell viability, respiratory burst and gene expression of Nrf2-Keapi signaling molecules in the peripheral blood leukocytes of blunt snout bream (Megalobrama amblycephala)

文献类型： 外文期刊

第一作者： Zhao, Zhenxin

作者： Zhao, Zhenxin;Xie, Jun;Liu, Bo;Ge, Xianping;Song, Changyou;Zhang, Huimin;Shan, Fan;Yang, Zhenfei;Xie, Jun;Liu, Bo;Ge, Xianping;Ren, Mingchun;Zhou, Qunlan;Miao, Linghong;Zhao, Zhenxin;Xie, Jun;Liu, Bo;Ge, Xianping;Song, Changyou;Ren, Mingchun;Zhou, Qunlan;Miao, Linghong;Zhang, Huimin;Shan, Fan;Yang, Zhenfei

作者机构：

关键词： Emodin; Nrf2 signaling pathway; Gene expression; Antioxidant defenses; Megalobrama amblycephala

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2017 年 62 卷

页码：

收录情况： SCI

摘要： We determined the effects of emodin on the cell viability, respiratory burst activity, mRNA levels of antioxidative enzymes (Cu-Zn SOD, CAT and NOX2), and gene expressions of the Nrf2-Keap1 signaling molecules in the peripheral blood leukocytes of blunt snout bream. Triplicate groups of cultured cells were treated with different concentrations of emodin (0.04-25 mu g/ml) for 24 h. Results showed that the emodin caused a dramatic loss in cell viability, and occurred in a dose-dependent manner. Emodin exposure (1-25 mu g/ml) were significantly induced the ROS generation compared to the control. The respiratory burst and NADPH oxidase activities were significantly induced at a concentration of 0.20 mu g/ml, and inhibited at 25 mu g/ml. Besides, mRNA levels of antioxidant enzyme genes were dramatically regulated by emodin exposure for 24 h. During low concentrations of exposure, mRNA levels of Cu-Zn SOD in the cells treated with 0.04, 0.20 mu g/ml, CAT, NOX2 and Nrf2 in the cells treated with 1 mu g/ml were sharply increased, respectively. Whereas, high concentrations were dramatically down -regulated the gene expressions of CAT in the cells treated with 5, 25 mu g/ml and NOX2 in the cells treated with 25 mu g/ml. Furthermore, sharp increase in Keapland Bach1 expression levels were observed a dose -dependent manner. In conclusion, this study demonstrated that emodin could induce antioxidant defenses which were involved in cytotoxic activities, respiratory burst and the transcriptional regulation levels of antioxidant enzymes and Nrf2-Keapl signaling molecules. (C) 2017 Elsevier Ltd. All rights reserved.

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