Prevalence and distribution of covert mortality nodavirus (CMNV) in cultured crustacean

文献类型： 外文期刊

第一作者： Zhang, Qingli

作者： Zhang, Qingli;Xu, Tingting;Wan, Xiaoyuan;Liu, Shuang;Wang, Xiuhua;Li, Xiaoping;Dong, Xuan;Yang, Bing;Huang, Jie;Zhang, Qingli;Xu, Tingting;Liu, Shuang;Wang, Xiuhua;Li, Xiaoping;Yang, Bing;Huang, Jie;Zhang, Qingli;Xu, Tingting;Liu, Shuang;Li, Xiaoping;Huang, Jie

作者机构：

关键词： Viral covert mortality nodavirus; Prevalence; Distribution; Crustacean

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2017 年 233 卷

页码：

收录情况： SCI

摘要： An emerging covert mortality nodavirus (CMNV) was proved to be the infectious agent of shrimp viral covert mortality disease (VCMD). Prevalence and distribution of CMNV were investigated by using the methods of reverse transcription loop-mediated isothermal amplification (RT-LAMP), nested reverse transcription PCR, gene sequencing, histopathology, in situ RNA hybridization (ISH) and transmission electron microscope (TEM) in this study. RT-LAMP results showed that CMNV positive samples appeared in the cultured crustaceans including Litopenaeus vannamei, Fenneropenaeus chinensis, Marsupenaeus japonicas, Penaeus monodon, and Macrobrachium rosenbergii, and mostly distributed the coastal provinces in China. The prevalence rates of CMNV among the collected samples in 2013, 2014 and 2015 were 45.93% (130/283), 27.91% (84/301) and 20.85% (54/259), respectively. CMNV infection in M. japonicas and P. monodon was verified by ISH. The presence of CMNV particles were confirmed by TEM analysis in the CMNV positive samples diagnosed by RT-LAMP. The high prevalence and wide epidemic distribution of CMNV in this investigation revealed that it was necessary to pay close attention to the high risk of CMNV transmission in farmed crustaceans. (C) 2017 Elsevier B.V. All rights reserved.

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