文献类型： 外文期刊
第一作者： Wu, Ting
作者： Wu, Ting;Zhou, Fangfang;Zhang, Long;Wu, Ting;Zhou, Hang-Xu;Jin, Bi-Kun;Zhang, Long;Yang, Bing;Wu, Ting;Zhou, Hang-Xu;Jin, Bi-Kun;Zhang, Long;Yang, Bing;Tian, Jing;Tang, Shibing;Tian, Jing;Tang, Shibing;Zhou, Rong;Zhou, Rong;Huangfu, Shangwei;Chen, Hongli;Sablina, Anna;Zhou, Fangfang
作者机构：
关键词： Cross-linking mass spectrometry; Thioredoxin; IMAC; Direct protein interaction; Subcellular organelles
期刊名称：GENOME BIOLOGY （ 影响因子：9.4； 五年影响因子：16.3 ）
ISSN： 1474-760X
年卷期： 2025 年 26 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundIt is crucial to investigate protein functions in specific subcellular environments. Cross-linking mass spectrometry is a powerful tool to map the direct interactome of proteins by identifying inter-protein cross-links. However, it is challenging to identify inter-protein cross-linked peptides due to their low abundance.ResultsWe chemically synthesize the cross-linkers ePDES1 and ePDES2 with an alkyne group and a compound with azide linked to a phosphate group to enrich for cross-linked peptides.ConclusionBased on the high-quality cross-linking spectra of ePDES1 and ePDES2, our methods achieve the identification of hundreds of directly interacting proteins or substrates of thioredoxins in the nucleus and mitochondria.
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