Interactive effect of ammonia and crowding stress on ion-regulation and expression of immune-related genes in juvenile turbot (Scophthalmus maximus)

文献类型： 外文期刊

第一作者： Liu, Bao-Liang

作者： Liu, Bao-Liang;Huang, Bin;Lei, Ji-Lin;Jia, Rui

作者机构：

关键词： Ammonia; immune response; ions; Scophthalmus maximus

期刊名称：MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY （ 影响因子：0.891； 五年影响因子：1.344 ）

ISSN： 1023-6244

年卷期： 2017 年 50 卷 3 期

页码：

收录情况： SCI

摘要： The present study was to investigate the interactive effect of ammonia and crowding stress on ion-regulation and expression of immune-related genes in juvenile turbot (Scophthalmus maximus). The fish were exposed to four total ammonia nitrogen (0, 5, 20, 40 mg/L TAN) and two stocking density. After 96 h of exposure, blood, gill, and liver samples were collected to measure biochemical parameters and mRNA levels of immune-related genes. The results showed that co-exposure to high TAN (20 and 40 mg/L) and high density significantly increased plasma sodium (Na+), gill Na+/K+-ATPase activity and mRNA levels. Following individual and combined exposure to high TAN and high density, the heat shock protein 70 (HSP 70), HSP 90, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) genes expression were obviously higher than their control. Conversely, the lysozyme (LZM) and hepcidin mRNA levels were down-regulated in liver of fish exposed to high TAN alone and combination of high TAN-density. Moreover, glutathione S-transferase (GST) mRNA levels significantly increased in treatments with individual high TAN and high density, but decreased in high TAN-density co-exposed fish. Overall, ion homeostasis and immune status were adversely influenced in high exposed turbot under high density.

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