Topoisomerase Mutations are Associated with High-Level Ciprofloxacin Resistance in Staphylococcus saprophyticus, Enterococcus faecalis and Escherichia coli Isolated from Ducks

文献类型： 外文期刊

第一作者： Ji, Guo

作者： Ji, Guo;Chen, Qiwei;Gong, Xiaowei;Zheng, Fuying;Li, Shengdou;Liu, Yongsheng

作者机构：

关键词： Ciprofloxacin; DNA gyrase; Mutation; Topoisomerase IV

期刊名称：PAKISTAN VETERINARY JOURNAL （ 影响因子：1.318； 五年影响因子：0.954 ）

ISSN： 0253-8318

年卷期： 2018 年 38 卷 1 期

页码：

收录情况： SCI

摘要： This study investigated the effects of ciprofloxacin on specie and resistance of the bacteria isolated from the ducks reared in ciprofloxacin-present and ciprofloxacin-free duck population. Bacteria were isolated from the carcass swab samples collected from the slaughtered ducks. The result showed that the dominant bacteria isolated from the ducks were Macrococcus caseolyticus (M. caseolyticus), Staphylocuccus saprophyticus (S. saprophyticus), Enterococcus faecalis (E. faecalis) and Escherichia coli (E. coli). The minimum inhibitory concentrations (MICs) of ciprofloxacin against all isolates were measured, and the genes of topoisomerase II (DNA gyrase) and topoisomerase IV of S. saprophyticus, E. faecalis and E. coli were amplified and sequenced, respectively. The results showed that the site-directed mutations of DNA gyrase and topoisomerase IV of the above three bacteria caused the strains to generate high-level resistance to ciprofloxacin (MIC >= 32 mu g/ml). No plasmid-mediated quinolone resistance (PMQR) genes were detected. These results suggested that ciprofloxacin could affect bacterial species in the duck and induce bacterial resistance. Site-directed mutations of DNA gyrase and topoisomerase IV of S. saprophyticus, E. faecalis and E. coli were closely related to high-level ciprofloxacin resistance. (c) 2017 PVJ. All rights reserved

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