Changes in degradation and phosphorylation level of titin in three ovine muscles during postmortem

文献类型： 外文期刊

第一作者： Wang, Ying

作者： Wang, Ying;Li, Xin;Li, Zheng;Li, Meng;Zhang, Dequan;Wang, Ying;Zhu, Jie;Wang, Ying;Zhu, Jie;Zhu, Jie

作者机构：

关键词： Degradation; MFI; pH; phosphorylation; titin

期刊名称：INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY （ 影响因子：3.713； 五年影响因子：3.408 ）

ISSN： 0950-5423

年卷期： 2018 年 53 卷 4 期

页码：

收录情况： SCI

摘要： The objective of this study was to investigate the degradation of titin and its phosphorylation level in three muscles from sheep. The MFI and pH from the longissimus lumborum (LL), semimembranosus (SM) and psoas major (PM) muscles were measured at 30min, 1, 2, 7, 14, 21 and 28days postmortem. Myofibrillar proteins were extracted, separated by SDS-PAGE and quantified by phosphor-specific staining. Phosphorylation of titin was predicted by Pro-Q Diamond-SYPRO Ruby staining. Two days after exsanguination, the pH and MFI of the PM were higher than those of the LL and SM muscles (P<0.05). The sarcomere length of the PM muscle was also longer than that of the LL and SM muscle (P<0.05). PM muscles had a highest phosphorylation level (P<0.05) at 0.5h postmortem and showed the greatest degree of titin degradation over 28days. This suggests that phosphorylation of titin might accelerate its degradation.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序