Neonatal respiratory distress syndrome and underlying mechanisms in cloned cattle

文献类型： 外文期刊

第一作者： Rao, Yifan

作者： Rao, Yifan;Zhang, Fanyi;Du, Weihua;Hao, Haisheng;Zhao, Xueming;Jiang, Qiuling;Liu, Yan;Sun, Xiuzhu;Jiang, Xiaojing;Huang, Linhua;Yang, Na;Guo, Xiaogai;Zhang, Fanyi

作者机构：

关键词： bovine; clone; neonatal; NRDS; reprogramming; somatic cell nuclear transfer

期刊名称：MOLECULAR REPRODUCTION AND DEVELOPMENT （ 影响因子：2.609； 五年影响因子：3.47 ）

ISSN： 1040-452X

年卷期： 2018 年 85 卷 3 期

页码：

收录情况： SCI

摘要： Neonatal respiratory distress is a major mortality factor in cloned animals, but the pathogenesis of this disease is rarely investigated. In this study, four neonatal cloned cattle, born after full-term gestation, exhibited symptoms of neonatal respiratory distress syndrome (NRDS), which included symptoms of hyaline membrane disease as well as disordered surfactant homeostasis in their collapsed lungs. No differences in DNA methylation or histone modifications correlated with the suppressed SPB and SPC transcription observed in the cloned cattle group (p>0.05), whereas TTF-1 occupancy at SPB and SPC promoter regions in cloned cattle was significantly reduced to 24% and 20% that of normal lungs, respectively (SPB, p<0.05; SPC, p<0.01). Decreased TTF1 expression, dysregulation of SPB and SPC transcription by TTF-1, and disordered proteolytic processing of Surfactant protein B precursor together potentially contribute to the disruption of surfactant homeostasis and NRDS in bovine clones. Elucidation of the associated mechanisms should facilitate the development of novel preventive or therapeutic strategies to reduce the mortality rate of cloned animals and to improve the efficiency of SCNT technology.

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