O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli

文献类型： 外文期刊

第一作者： Bao, Yinli

作者： Bao, Yinli;Ma, Jiale;Li, Ganwu;Bao, Yinli;Zhang, Haobo;Ma, Jiale;Huang, Xinxin;Logue, Catherine M.;Nolan, Lisa K.;Li, Ganwu

作者机构：

关键词： Extraintestinal pathogenic E coli (ExPEC); neonatal meningitis-associated E coli (NMEC); lysozyme resistance; lipopolysaccharide (LPS); O-specific polysaccharide

期刊名称：VIRULENCE （ 影响因子：5.882； 五年影响因子：6.489 ）

ISSN： 2150-5594

年卷期： 2018 年 9 卷 1 期

页码：

收录情况： SCI

摘要： Extraintestinal pathogenic Escherichia coli (ExPEC) is the leading cause of bloodstream and other extraintestinal infections in human and animals. The greatest challenge encountered by ExPEC during an infection is posed by the host defense mechanisms, including lysozyme. ExPEC have developed diverse strategies to overcome this challenge. The aim of this study was to characterize the molecular mechanism of ExPEC resistance to lysozyme. For this, 15,000 transposon mutants of a lysozyme-resistant ExPEC strain NMEC38 were screened; 20 genes were identified as involved in ExPEC resistance to lysozyme-of which five were located in the gene cluster between galF and gnd, and were further confirmed to be involved in O-specific polysaccharide biosynthesis. The O-specific polysaccharide was able to inhibit the hydrolytic activity of lysozyme; it was also required by the complete lipopolysaccharide (LPS)-mediated protection of ExPEC against the bactericidal activity of lysozyme. The O-specific polysaccharide was further shown to be able to directly interact with lysozyme. Furthermore, LPS from ExPEC strains of different O serotypes was also able to inhibit the hydrolytic activity of lysozyme. Because of their cell surface localization and wide distribution in Gram-negative bacteria, O-specific polysaccharides appear to play a long-overlooked role in protecting bacteria against exogenous lysozyme.

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