Production of Transgenic Mice Through Sperm-Mediated Gene Transfer Using Magnetic Nano-Carriers

文献类型： 外文期刊

第一作者： Wang, Yan

作者： Wang, Yan;Zhao, Xiang;Du, Wei;Chen, Wenjie;Sun, Changjiao;Cui, Bo;Zeng, Zhanghua;Shen, Yue;Gao, Fei;Wang, Anqi;Liu, Guoqiang;Cui, Haixin;Wang, Yan;Zhao, Xiang;Du, Wei;Sun, Changjiao;Cui, Bo;Zeng, Zhanghua;Shen, Yue;Gao, Fei;Wang, Anqi;Liu, Guoqiang;Cui, Haixin;Liu, Jinghao;Chen, Wenjie

作者机构：

关键词： Sperm-Mediated Gene Transfer; Magnetic Nanoparticles; Mice; Carriers; Plasmid DNA

期刊名称：JOURNAL OF BIOMEDICAL NANOTECHNOLOGY （ 影响因子：4.099； 五年影响因子：3.492 ）

ISSN： 1550-7033

年卷期： 2017 年 13 卷 12 期

页码：

收录情况： SCI

摘要： Current methods of transgenic animal production suffer from low efficiency, cumbersome operation, and high cost. Magnetic nanoparticles (MagNPs) have several characteristics, such as a high carrying efficiency, non-immunogenicity, and strong targeting inducible via magnetic fields, that make them well-suited for use in the generation of transgenic animals. In this study, we used magnetic nano-carriers combined with sperm-mediated gene transfer (SMGT) to generate transgenic mice that harbor the enhanced green fluorescent protein (EGFP) gene. Exogenous plasmid DNA loaded onto Fe3O4 MagNPs were first delivered into mouse sperm cells under a magnetic field. Transfected sperm cells were then incubated with oocytes to complete fertilization, and transgenic mice were successfully generated though embryo transplantation. We demonstrate that this method is exceedingly facile, fast, and cost-effective, with higher transfection efficiency than that of conventional liposome methods.

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