Gold Nanoparticle-Decorated Single-Walled Carbon Nanotubes as a Catalytic Amplification Platform for the Electrochemical Detection of Alkaline Phosphatase Activity

文献类型： 外文期刊

第一作者： Zhao, Lingzhi

作者： Zhao, Lingzhi;Miao, Yanqing;Zhao, Lingzhi;Zhang, Chenxiao;Zhao, Liu

作者机构：

关键词： Alkaline phosphatase activity; Gold particles; Single-walled carbon nanotubes (SWNTs); whole cell lysate

期刊名称：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE （ 影响因子：1.765； 五年影响因子：1.685 ）

ISSN： 1452-3981

年卷期： 2018 年 13 卷 2 期

页码：

收录情况： SCI

摘要： The detection of alkaline phosphatase (ALP) activity is of great significance in many biomedical applications and for understanding the functional mechanism of ALP-related biological events. A sensitive electrochemical ALP biosensor using ascorbic acid 2-phosphate (AA-P) as the substrate was developed based on a gold nanoparticle-decorated single-walled carbon nanotube (GNP/SWNT)-modified glassy carbon electrode (GCE). The activity of ALP was determined indirectly according to the concentration of ascorbate (AA), which was generated during the hydrolysis reaction of AA-P in the presence of ALP as a catalyst. The biosensor exhibited a low applied potential, high sensitivity and selectivity, and the current response increased with ALP concentration from 3 to 50 U L-1 with a detection limit of 0.2 U L-1. The applicability of the developed method was demonstrated for successfully detecting ALP in whole HeLa cell lysate, thus providing a promising tool for other sensing systems that involve ALP.

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