Polysaccharide structure and immunological relationships of RG-I pectin from the bee pollen of Nelumbo nucifera

文献类型： 外文期刊

第一作者： Li, Shanshan

作者： Li, Shanshan;Yang, Guang;Yan, Jingmin;Wu, Di;Hou, Yu;Zhou, Yifa;Li, Shanshan;Diao, Qiyu

作者机构：

关键词： Nelumbo nucifera bee pollen; RG-I pectin; Structural-immunological activity relationship

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2018 年 111 卷

页码：

收录情况： SCI

摘要： From the bee pollen of Nelumbo nucifera, we used hot water extraction and chromatographic fractionation on DEAE-cellulose and Sepharose CL-6B to purify polysaccharides (WNPP) to homogeneity. WNPP-2-RG (3.8 x 10(5) Da) consisted primarily of Rha (11.5%), GalA (12.0%), Gal (412%) and Ara (29.7%). Structure analysis showed that this polysaccharide is a RG-I type pectin containing AG-I and AG-II side chains comprised of 1,5-L-Ara (25.6%), t-D-Gal (12.0%), and 1,6-D-Gal (18.3%) as the primary linkage types. Immunological activity assays demonstrated that WNPP-2-RG increased lymphocyte proliferation and macrophage phagocytosis, and induced production of NO. Compared to WNPP-2-RG, its hydrolysates (RG-8H-P) had no effect on lymphocyte proliferation, although they did promote macrophage phagocytosis and decreased NO production. Our results suggest that Gal side chain residues may play a crucial role in macrophage phagocytosis, whereas Ara is unimportant for NO production and neither Gal nor Ara impact lymphocyte proliferation. (C) 2018 Elsevier B.V. All rights reserved.

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