Genomic structure and molecular characterization of growth hormone and its expression response to different feed types in golden pompano Trachinotus ovatus (Linnaeus, 1758)

文献类型： 外文期刊

第一作者： Liang, Yin-Yin

作者： Liang, Yin-Yin;Guo, Hua-Yang;Zhu, Ke-Cheng;Zhang, Nan;Yang, Jing-Wen;Sun, Xiao-Xiao;Jiang, Shi-Gui;Zhang, Dian-Chang;Liang, Yin-Yin;Sun, Xiao-Xiao;Guo, Hua-Yang;Zhu, Ke-Cheng;Zhang, Nan;Yang, Jing-Wen;Zhang, Dian-Chang;Guo, Hua-Yang;Zhu, Ke-Cheng;Zhang, Nan;Yang, Jing-Wen;Zhang, Dian-Chang

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关键词： expression regulation; feed type; genomic structure; growth hormone; Trachinotus ovatus

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN： 1355-557X

年卷期： 2018 年 49 卷 5 期

页码：

收录情况： SCI

摘要： Growth hormone and feed types all play important roles in the growth of vertebrates. In this study, to investigate molecular characterization of the Trachinotus ovatus growth hormone (ToGH) and its expression in response to the different feed types, we characterized the genomic structure of GH and analysed expression patterns using quantitative real-time PCR (qRT-PCR). ToGH spans approximately 4.0kb and consists of six exons and five introns, with 606bp open reading frame (ORF). The 5-flanking region contains consensus sequences for three TATA boxes, one pit-1a, three GRE, two TRE, two HNF-3 and two ERE. A phylogenetic analysis of Percoidei confirmed that the phylogenetic trees based on the GH gene are reliable at the genera level. The expression of ToGHmRNA was highest in intestine, brain and muscle and lowest in liver, spleen and kidney (p<.05). The dietary experiment demonstrated that the transcription level of ToGH in T.ovatus fed with iced fish was higher than in those fed with iced squid and pelleted feed (p<.05). Fish fed with pelleted feed had higher ToGH expression in their intestine than those fed iced squid (p<.05); however, ToGH expression in fish fed with iced fish did not significantly differ from the other feeding groups. This study would be useful to understanding the potential function of GH gene in out pituitary tissues by diets and providing a certain strategy for feed research.

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