Soybean hairy roots produced in vitro by Agrobacterium rhizogenes-mediated transformation

文献类型： 外文期刊

第一作者： Chen, Li

作者： Chen, Li;Cai, Yupeng;Liu, Xiujie;Guo, Chen;Hou, Wensheng;Chen, Li;Cai, Yupeng;Liu, Xiujie;Guo, Chen;Sun, Shi;Wu, Cunxiang;Jiang, Bingjun;Han, Tianfu;Hou, Wensheng

作者机构：

关键词： Agrobacterium rhizogenes; Hairy root; Protocol; Soybean; Transformation

期刊名称：CROP JOURNAL （ 影响因子：4.407； 五年影响因子：5.687 ）

ISSN： 2095-5421

年卷期： 2018 年 6 卷 2 期

页码：

收录情况： SCI

摘要： Soybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro. Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants. After infection and co-cultivation, hairy roots were produced in induction culture medium after 10-12 days. Using this method, 90%-99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%-60% of the hairy roots induced were transformed. Based on high transformation efficiency and short transformation period, this method represents an efficient and rapid platform for study of soybean gene function. (C) 2017 "Crop Science Society of China and Institute of Crop Science, CAAS". Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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