文献类型： 外文期刊
第一作者： Wu, Yangnan
作者： Wu, Yangnan;Guan, Weijun;Zheng, Yanjie;Ji, Hongda;Wang, Xishuai;Wen, Hebao;Ren, Han
作者机构：
关键词： Heart-Derived Mesenchymal Stem Cells; Goose; Isolation; Biological Characterization
期刊名称：JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING （ 影响因子：0.287； 五年影响因子：0.435 ）
ISSN： 2157-9083
年卷期： 2018 年 8 卷 2 期
页码：
收录情况： SCI
摘要： Objective: This research is designed to establish the system of isolation and culture for goose heart-derivedmesenchymal stem cells, explore its biological characteristics and differentiation ability in vitro. Methods: Heart-derived mesenchymal stem cells were isolated by enzymatic digestion. The specific surface markers of heart-derived mesenchymal stem cells were detected by reverse transcription-polymerase chain reaction, immunofluorescence and flow cytometry. Growth curve was drew to analyze cell viability. Oil Red O staining, alizarin red staining, alcian blue staining and reverse transcription-polymerase chain reaction assay were used to detect differentiation of the heart-derived mesenchymal stem cells. Results: Heart-derived mesenchymal stem cells were obtained successfully and sub-cultured to passage 22 in vitro. The expression of specific surface markers on cells were positive. The growth curve appeared in typical S curve. Furthermore, cells were induced to differentiate into adipocytes, osteoblast, and chondrocyte successfully. Conclusion: Heart-derived mesenchymal stem cells can be acquired from goose embryo heart and have shown strong self-renewal capacity and multipotential differentiation ability in vitro. The results denote that heart-derived mesenchymal stem cells can be an ideal seeded cells for clinical treatment and tissue engineering.
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