Evaluation of the protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing BmAMA1 as vaccines against Babesia microti infection in hamster

文献类型： 外文期刊

第一作者： Wang, Guanbo

作者： Wang, Guanbo;Efstratiou, Artemis;Moumouni, Paul Franck Adjou;Liu, Mingming;Guo, Huanping;Gao, Yang;Li, Jixu;Ringo, Aaron Edmond;Xuan, Xuenan;Yu, Longzheng;Cao, Shinuo;Zhou, Mo

作者机构：

关键词： Babesia microti; DNA vaccine; Adenovirus; Heterologous prime-boost; BmAMA1

期刊名称：ACTA PARASITOLOGICA （ 影响因子：1.44； 五年影响因子：1.335 ）

ISSN： 1230-2821

年卷期： 2018 年 63 卷 2 期

页码：

收录情况： SCI

摘要： In the present study, we have investigated the protective effect of a heterologous prime-boost strategy with priming plasmid DNA followed by recombinant adenovirus, both expressing BmAMA1, against Babesia microti infection. Four groups consisting of 3 hamsters per group were immunized with pBmAMA1/Ad5BmAMA1, pNull/Ad5BmAMA1, pBmAMA1/Ad5Null and pNull/Ad5Null, followed by challenge infection with B. microti. Our results showed that hamsters immunized with plasmid and adenovirus expressing BmAMA1 developed a robust IgG and IgG2a antibody response against BmAMA1, suggesting the DNA vaccine or viral vector vaccine tend to induce a Th1-biased response. Compared to the control hamsters, the hamsters vaccinated either with the prime-boost strategy or one of the two "vaccines" exhibited no significant protection against B. microti challenge. Although a slight difference in terms of parasitemia and hematocrit values at days 14-16 post challenge infection was observed, no other statistical difference was detected. Our results indicate that the prime-boost vaccination strategy of injection of plasmid and adenovirus expressing BmAMA1 is not efficient in protecting against B. microti infection.

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