Jasmonate-responsive MYB factors spatially repress rutin biosynthesis in Fagopyrum tataricum

文献类型： 外文期刊

第一作者： Zhang, Kaixuan

作者： Zhang, Kaixuan;Wan, Dongpu;Li, Long;Zhou, Meiliang;Logacheva, Maria D.;Meng, Yu;Hu, Jianping;Wan, Dongpu;Yu, Zhuo;Li, Long;Yan, Mingli;Janovska, Dagmar;Wang, Zhiyong;Georgiev, Milen I.;Georgiev, Milen I.;Yang, Fuyu

作者机构：

关键词： 26S proteasome; buckwheat; Fagopyrum tataricum; jasmonates; MYB transcription factor; phenylpropanoid pathway

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2018 年 69 卷 8 期

页码：

收录情况： SCI

摘要： Jasmonates are plant hormones that induce the accumulation of many secondary metabolites, such as rutin in buckwheat, via regulation of jasmonate-responsive transcription factors. Here, we report on the identification of a clade of jasmonate-responsive subgroup 4 MYB transcription factors, FtMYB13, FtMYB14, FtMYB15, and FtMYB16, which directly repress rutin biosynthesis in Fagopyrum tataricum. Immunoblot analysis showed that FtMYB13, FtMYB14, and FtMYB15 could be degraded via the 26S proteasome in the COI1-dependent jasmonate signaling pathway, and that this degradation is due to the SID motif in their C-terminus. Yeast two-hybrid and bimolecular fluorescence complementation assays revealed that FtMYB13, FtMYB14, and FtMYB15 interact with the importin protein Sensitive to ABA and Drought 2 ( FtSAD2) in stem and inflorescence. Furthermore, the key repressor of jasmonate signaling FtJAZ1 specifically interacts with FtMYB13. Point mutation analysis showed that the conserved Asp residue of the SID domain contributes to mediating protein-protein interaction. Protoplast transient activation assays demonstrated that FtMYB13, FtMYB14, and FtMYB15 directly repress phenylalanine ammonia lyase ( FtPAL) gene expression, and FtSAD2 and FtJAZ1 significantly promote the repressing activity of FtMYBs. These findings may ultimately be promising for further engineering of plant secondary metabolism.

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