Genetically engineering Crambe abyssinicaA potentially high-value oil crop for salt land improvement

文献类型： 外文期刊

第一作者： Qi, Weicong

作者： Qi, Weicong;Shao, Hongbo;Qi, Weicong;Tinnenbroek-Capel, Iris E. M.;Salentijn, Elma M. J.;Cheng, Jihua;Visser, Richard G. F.;Krens, Frans A.;Van Loo, Eibertus N.;Qi, Weicong;Zhang, Zhao;Huang, Bangquan;Cheng, Jihua

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关键词： Crambe abyssinica; lysophosphatidic acid acyltransferase; metabolism pathway regulation; oil; salt land improvement

期刊名称：LAND DEGRADATION & DEVELOPMENT （ 影响因子：4.977； 五年影响因子：5.291 ）

ISSN： 1085-3278

年卷期： 2018 年 29 卷 4 期

页码：

收录情况： SCI

摘要： Crambe abyssinica (crambe) is a new industrial oil crop that can grow on saline soil and tolerates salty water irrigation. Genetically engineered crambe in which the seed-oil composition is manipulated for more erucic acid and less polyunsaturated fatty acid (PUFA) would be highly beneficial to industry. In this research, lysophosphatidic acid acyltransferase 2 RNA interference (CaLPAT2-RNAi) was introduced into the crambe genome to manipulate its oil composition. The result showed in comparison with wild type, CaLPAT2-RNAi could significantly reduce linoleic and linolenic acid content, simultaneously increasing erucic acid content. Systematic metabolism engineering was then carried out to further study CaLPAT2-RNAi, combined with the overexpression of Brassica napusfatty acid elongase (BnFAE), Limnanthes douglasii LPAT (LdLPAT), and RNAi of endogenous fatty acid desaturase 2 (CaFAD2-RNAi). Oil composition analysis on the tranformants' seeds showed that (a) with CaFAD2-RNAi, PUFA content could be dramatically decreased, in comparison with BnFAE+LdLPAT+CaFAD2-RNAi, and BnFAE+LdLPAT+CaFAD2-RNAi+CaLPAT2-RNAi seeds showed lower linolenic acid content; (b) BnFAE+LdLPAT+CaFAD2-RNAi+CaLPAT2-RNAi could increase the erucic acid content in crambe seed oil from less than 66.6% to 71.6%, whereas the highest erucic acid content of BnFAE+LdLPAT+CaFAD2-RNAi was 79.2%; (c) although the four-gene combination could not increase the erucic acid content of seed oil to a higher level than the others, it led to increased carbon resource deposited into C22:1 and C18:1 moieties and lower PUFA. Summarily, the present research indicates that suppression of LPAT2 is a new, promising strategy for seed-oil biosynthesis pathway engineering, which would increase the value of crambe oil.

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