Heterotrimeric G-Protein gamma Subunit CsGG3.2 Positively Regulates the Expression of CBF Genes and Chilling Tolerance in Cucumber

文献类型： 外文期刊

第一作者： Bai, Longqiang

作者： Bai, Longqiang;Liu, Yumei;Mu, Ying;Anwar, Ali;He, Chaoxing;Yan, Yan;Li, Yansu;Yu, Xianchang;Liu, Yumei

作者机构：

关键词： G-protein gamma subunit; CsGG3; cucumber; cold stress; CBF genes

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： Heterotrimeric guanine nucleotide-binding proteins (G proteins) composed of alpha (G alpha), beta (G beta), and gamma (G gamma) subunits are central signal transducers mediating the cellular response to multiple stimuli, such as cold, in eukaryotes. Plant G g subunits, divided into A, B, and C three structurally distinct types, provide proper cellular localization and functional specificity to the heterotrimer complex. Here, we demonstrate that a type C G g subunit CsGG3.2 is involved in the regulation of the CBF regulon and plant tolerance to cold stresses in cucumber (Cucumis sativus L.). We showed that CsGG3.2 transcript abundance was positively induced by cold treatments. Transgenic cucumber plants (T1) constitutively over-expressing CsGG3.2 exhibits tolerance to chilling conditions and increased expression of CBF genes and their regulon. Antioxidative enzymes, i.e., superoxide dismutase, catalase, peroxidase, and glutathione reductase activities increased in cold-stressed transgenic plants. The reactive oxygen species, oxygen free radical and H2O2, production, as well as membrane lipid peroxidation (MDA) production decreased in transgenic plants, suggesting a better antioxidant system to cope the oxidative-damages caused by cold stress. These findings provide evidence for a critical role of CsGG3.2 in mediating cold signal transduction in plant cells.

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